胰腺癌干细胞中胚胎干性相关基因的表达及意义

摘要

Objective To observe the expression change of embryonic dry related genes in embryonic stem of pancreatic cancer and analyze its significance.Methods Pancreatic cancer cell lines PANC-1 was incubated with with CD24,CD44 antibody incubation,the CD24 + and CD44 +pancreatic cancer stem cells were sorted and pancreatic cancer stem cells' forming speed were observed under a microscope.The differentiation of stem cells were induced by 1640 culture,and the expression of CD24 +and CD44 +were detected by flow cytometry.The expression of embryonic stem cell marker Oct4 and Nano were detected by immunohistochemical and the tolerance difference of Oct4 and Nano cell to chemotherapeutic drugs in vitro were observed and compared by CCK8 method.Results The number of CD24 +and CD44 +isolated from human pancreatic cancer cell lines PANC-1 was 1%~3%.Oct4 and Nano expression in sorted cells were significantly higher than un -sorted cells,and the difference was significant (P<0.05 ). Conclusion CD24 +and CD44 +cells has high drug resistance and other characteristics of pancreatic cancer stem cells,and the expression of embryonic stem-related genes Oct4 and Nano are high pancreatic cancer stem cells.%目的：观察和分析胰腺癌干细胞中胚胎干性相关基因的表达变化及意义。方法以中科院细胞库人胰腺癌细胞株PANC-1为研究对象，将其配置成单细胞悬液，与CD24、CD44等进行抗体孵育，分选出CD24＋、CD44＋等胰腺癌干细胞，显微镜下观察胰腺癌干细胞的形成速度；采用1640培养液诱导干细胞球细胞进行分化，流式检测CD24＋、CD44＋表达。免疫组化法检测胚胎干细胞标志物Oct4和Nano的表达，CCK8法比较Oct4和Nano细胞化疗药物的体外耐药差别。结果从人胰腺癌细胞株PANC-1分离出的CD24＋、CD44＋的胰腺癌干细胞在1％～3％之间，Oct4和Nano在分选出的细胞中表达明显高于未分选组，差异具有统计学意义（P＜0.05）。结论 CD24＋、CD44＋细胞具有胰腺癌干细胞的高耐药性等特性，且Oct4和Nano等胚胎干性相关基因在胰腺癌干细胞中表达性高。