目的：探讨大蒜素对Wister鼠模型中腺窝异常病灶（abnormal crypt focus，ACF）的影响。方法60只Wister大鼠，按照20 mg/kg皮下注射二甲肼（dimethylhydrazine，DMH），每周1次，连续喂养18周。随机分为大蒜素组和非大蒜素组，将每组分为3批，分别在开始用药后8周、16周与24周分别处死大鼠，每批10只，美兰染色后镜片下观察大肠组织。结果大肠被均等分为10份进行观察，ACF大部分分布于中段和远端，大约在50%～80%处。非大蒜素组的基本情况：8周ACF平均（63.97±1.22）个/只，16周ACF平均（83.97±1.13）个/只，24周ACF平均（69.33±2.01）个/只；大蒜素组的基本情况：8周ACF平均（9.4±1.12）个/只，16周ACF平均（7.17±1.33）个/只，24周ACF平均（4.97±1.23）个/只。非大蒜素组ACF的发生情况显著高于大蒜素组，2组ACF发生率和数量差异有统计学意义（χ2=15.88，P<0.01）。结论大蒜素可以明显降低Wister大鼠腺窝异常病灶的发生数量，在一定程度上降低直肠癌的发生率，起到预防的作用。%Objective To study the inlfuence of the allicin on abnormal crypt focus(ACF). in Wister rat model. Methods 60 Wister rats were subcutaneous injected by dimethylhydrazine (DMH), according to 20 mg/kg weight, once a week, feeding 18 weeks continuously. All of the rats were randomly divided into allicin group and non-allicin group, each group was divided into three groups evenly, and put to death at 8, 16 and 24 weeks after medication separately. Large intestines were observed after dyeing. Results Large intestines were divided into ten equally, and all pieces were observed. ACF were almostly distributed in the middle and distal of the large intestines, about in 50%-80%.The basic situation of non-allicin group was as follows:ACF at 8 weeks was 63.97±1.22 on average per each, 16 weeks was 83.97±1.13, 24 weeks was 69.33±2.01. The basic situation of allicin group:ACF at 8 weeks was 9.4±1.12 on average per each , 16 weeks was 7.17±1.33, 24 weeks was 4.97±1.23.The occurrence of ACF in non-allicin group was higher than allicin group, the differences of incidence rate and number of ACF between two groups was significant (χ2=15.88,P<0.01).Conclusion Allicin can significantly reduce the number of ACF in Wister rats, lower the incidence of colorectal cancer, has prevention effect.
展开▼
