枸橼酸咖啡因对睡眠呼吸暂停综合症大鼠肺功能、VEGF与胰岛素样生长因子影响研究

摘要

Objective To analysis the effect of caffeine citrate on pulmonary function, vascular endothelial growth factor (VEGF)and insulin like growth factor -1 (IGF-1) in the apnea syndrome rats.Methods 80 male Wistar rats were selected, 20 were randomly selected to be the control group, the rest of the rats were replicated of apnea syndrome model.The rats were randomly divided into model group, experiment group and positive drug group, 20 of each group.The experimental group was given caffeine citrate injection of 5 mg/kg intraperitoneal injection, the positive drug group was given intraperitoneal injection of aminophylline 3 mg/kg, the model group was given intraperitoneal injection of normal saline, once a day, continuously for 1 week.Pulmonary function, serum VEGF, IGF-1 levels and sleep apnea were compared after the experiment.ResuIts Compared with the positive drug group, the related indexes of pulmonary function of the experimental group increased significantly ( P<0.05 ) .Serum VEGF levels decreased significantly (P<0.05).The serum IGF-1 level increased significantly (P<0.05).The sleep apnea index decreased significantly during the period of NREM and REM.(P<0.05).ConcIusion Caffeine citrate can improve apnea syndrome rats lung function, reduce the serum VEGF level, promote the formation of serum IGF-1, reduce the sleep apnea index.%目的：探讨枸橼酸咖啡因对睡眠呼吸暂停综合症大鼠肺功能、血管内皮细胞生长因子（ VEGF）与胰岛素样生长因子－1（ IGF－1）的影响。方法选取睡眠呼吸暂停综合症模型Wistar雄性大鼠80只，随机选取20只为正常对照组，其余进行呼吸暂停综合征模型复制，模型复制成功后，随机分为模型组20只，实验组20只和阳性药组20只，实验组予枸橼酸咖啡因注射液5 mg／kg腹腔注射，阳性药组给予氨茶碱3 mg／kg腹腔注射，模型组等量生理盐水腹腔注射，每天1次，连续1周；干预结束后，比较各组大鼠的肺功能相关指标、血清VEGF、IGF－1含量及睡眠呼吸暂停相关指标。结果与阳性药组比较，实验组大鼠肺功能相关指标明显升高（ P＜0．05）；血清VEGF水平明显降低（P＜0．05）；血清IGF－1水平明显升高（P＜0．05）；NREM期及REM期的睡眠呼吸暂停指数明显下降（ P＜0．05）。结论枸橼酸咖啡因能够改善睡眠呼吸暂停综合症大鼠的肺功能，降低其血清VEGF水平，促进血清IGF－1的生成，使睡眠呼吸暂停指数降低。