苦参素对大鼠实验性自身免疫性脑脊髓炎的研究

摘要

目的：建立大鼠实验性自身免疫性脑脊髓炎（ EAE）模型，观察其病理学改变；并探讨苦参素对其的影响。方法取Wistar大鼠30只，随机分为空白对照组、实验性自身免疫性脑脊髓炎模型组（以下简称模型组）和苦参素组。采用EAE症状评分标准对造模后大鼠进行评测，以观察其行为学变化；通过病理学HE染色和Kluver ＆ Barrera髓鞘染色，观察脑和脊髓炎症浸润、血管袖套改变和脊髓脱髓鞘变化。结果空白对照组动物未见行为学变化和病理学改变。模型组所有动物均出现行为学改变，并伴有髓鞘脱失和不同程度的脑和脊髓炎症浸润。苦参素组6只大鼠未出现EAE行为学变化的临床表现，且髓鞘结构基本完整。结论苦参素可以延长大鼠实验性自身免疫性脑脊髓炎的疾病潜伏期，缓解临床症状并保护神经。%Objective To establish experimental autoimmune cerebral spinal cord inflammation ( EAE) model rats, and observe the pathological changes and effect of oxymatrine on EAE model.Methods 30 rats were randomly divided into control group,EAE ( model group) group and oxymatrine group.The EAE symptom score was used to evaluate the rats after the model, and to observe the changes of its behavior.By HE staining and Kluver &Barrera myelin dyeing to observe the inflammation of the brain and spinal cord demyelinating changes.Results The animals in control group had no change in behavior and pathological.In model group, all animals occured behavioral changes, accompanied by varying degrees of demyelination and inflammatory infiltration of the brain and spinal cord.In oxymatrine group,6 rats did not appear EAE clinical manifestations and behavioral change, and the myelin structure was intact.Conclusion Oxymatrine can extend the incubation period experimental autoimmune encephalomyelitis in rats of the disease, relieve symptoms and protect nerve.