冠心十味丸对离体大鼠心肌缺血再灌注损伤后的保护作用

摘要

目的：研究冠心十味丸对离体大鼠心肌缺血再灌注损伤（MIRI）的保护作用。方法50只Wistar大鼠随机分成正常组、模型组、冠心十味丸低、高剂量组（0．3、0．9 mg／kg）、阳性对照组（复方丹参滴丸处理液），每组各10只。构建离体大鼠心脏再灌注实验模型，测定给药后70 min各组大鼠心肌组织中超氧化物歧化酶（ SOD）、乳酸脱氢酶（ LDH）、肌酸激酶（ CK）、丙二醛（ MDA）、Ca2＋－ATP酶、Ca2＋－Mg2＋－ATP酶、Na＋－K＋－ATP酶的含量。结果与模型组相比，冠心十味丸可明显升高心肌缺血再灌注损伤时SOD、Ca2＋－ATP酶、Ca2＋－Mg2＋－ATP酶、Na＋－K＋－ATP酶活性（ P＜0．05或P＜0．01），降低缺血再灌注损伤时LDH、CK活性，减少MDA含量（P＜0．05或P＜0．01）。结论冠心十味丸对离体大鼠心肌缺血；再灌注损伤具有保护作用。%Objective To investigate the protective effect of GuanXinShiWeiWan on ischemia reperfusion(I-R) injury rat.Methods 50 Wistar rats were randomly divided into normal group, model group, GuanXinShiWeiWan low ( 0.3 mg/kg ) and high ( 0.9 mg/kg ) dose group and positive group, each had 10 rats.Adopting the model of ischemia reperfusion injury of isolated rat hearts, the contents of SOD,MDA,LDH,CK and Ca2 +-ATP, Ca2 +-Mg2 +-ATP, Na +-K +-ATP in cardiac muscle tissue were tested and compared.Results Compared with model group, GuanXinShiWeiWan significantly improved the activities of SOD、Ca2 +-ATP,Ca2 +-Mg2 +-ATP and Na +-K +-ATP (P<0.05 or P<0.01) in cardiac muscle tissue which injured by ischemia reperfusion, while reducing markedly the contents of MDA,LDH,CK(P<0.05 or P<0.01).Conclusion GuanXinShiWeiWan can profect the cardiac muscle of ischemia reperfusion.