藏药'楚礁且莫'对消化系统肿瘤细胞体外抑制作用的实验研究

摘要

目的 探讨藏药"楚礁且莫"对人胃癌细胞MGC803、结直肠癌细胞LoVo及肝癌细胞HepG2的体外抑制作用.方法 将不同浓度的"楚礁且莫"提取物分别加入处于对数生长期的消化系统肿瘤细胞培养48 h,通过MTT实验检测"楚礁且莫"对各组肿瘤细胞的抑制作用,利用Hoechst 33342染色检测药物对肿瘤细胞凋亡的影响,并采用Elisa实验检测凋亡相关因子Bcl-2和Bax的水平.结果 MTT实验证实"楚礁且莫"能够显著抑制不同消化系统肿瘤细胞的增殖,荧光染色显示"楚礁且莫"对于3种常见消化道肿瘤细胞的凋亡均具有一定的促进作用,Elisa实验表明"楚礁且莫"能够上调肿瘤细胞促凋亡因子Bax的水平,同时下调抑制细胞凋亡的Bcl-2的水平.结论 藏药"楚礁且莫"能够在体外抑制消化系统肿瘤细胞的增殖,并促进其凋亡,其作用机制可能与Bcl-2家族蛋白相关.%Objective To investigate the inhibition effects of "Phrul sByor Chem Mo (PBCM)" against different common digestive system tumor cell lines in vitro, including human gastric carcinoma cell line MGC803, colorectal carcinoma cell line LoVo, and hepatocellular carcinoma cell line HepG2. Methods The digestive system tumor cell lines in the logarithmic growth phase were incubated with different concentrations of PBCM for 48h. The cell viability was investigated by MTT assays, apoptosis was detected by Hoechst 33342 assays, and the level of Bcl-2 and Bax was estimated by ELISA tests. Results MTT assays proved that the proliferation of digestive system tumor cell lines was significantly inhibited by PBCM in vitro. Fluorescent staining demonstrated that PBCM was able to promote apoptosis of the cell lines. ELISA tests illustrated that the level of proapoptotic Bax was elevated, while the value of antiapoptotic Bcl-2 was decreased. Conclusion PBCM is able to inhibit proliferation and induce apoptosis of digestive system tumor cell lines in vitro. The mechanism may be related with Bcl-2 family proteins.