Growth Inhibition of Breast Tumor Cells by Hypodense and Normodense Eosinophilic Cell Lines

摘要

These studies were based on the hypothesis that activated eosinophils release substances (mediators) that inhibit the growth of breast tumor cells in vitro. Cultured supernatants (24hr, 48hr and 72hr) from eosinophil cell lines were examined for growth inhibition of MCF-7 and MDA-MB- 231 breast tumor cell colony formation. Serial 2-fold dilutions (1:2, 1:4 and 1:8) inhibited both MCF- 7 and MDA-MB-23 1 colony formation in vitro. The time of harvesting of the supernatants affected their inhibitory capacity 72hr> 48hr> 24hr. Prior testing of eosinophil conditioned media revealed the presence of IL-4, and current examination revealed the presence of TNF alpha(>4Opg/ml). When exogenous cytokines were used, TNF alpha potently inhibited colony formation of MCF-7, while IL-3, IL-4, IL-S were marginal in their activity (10-35%) inhibitory activity.