灵芝胶囊对伴HPV感染宫颈癌患者肿瘤组织CD105蛋白及miR-21表达的影响

摘要

Objective To investigate the effect of Ganoderma capsule on the expression of CD105 protein and miR-21 in cervical cancer tumor tissue associated with human papillomavirus ( HPV) infection.Methods 162 patients with HPV positive cervical cancer from the department of oncology in Cixi City Hospital of traditional Chinese Medicine were selected and divided into two groups, 81 cases in the control group were treated with neoadjuvant chemotherapy, 81 cases in the experiment group received Ganoderma capsule on the basis of the control group , the expression levels of CD105 protein, vascular endothelial growth factor (VEGF), miR-21 in tumor tissues, microvessel formation correlation indexes, peripheral blood T lymphocyte levels, and the clinical effect were compared after the treatment.Results The control rate in the control group(72.84%)was lower than the experiment group(86.41%) (P<0.05).Compared with the control group, the expression levels of CD105 protein, VEGF in tumor tissue were lower in the experiment group after treatment, serum levels of CD105 protein,transforming growth factor-β1 (TGF-β1), VEGF were lower after chemotherapy, the expression level of miR-21 was lower after treatment, peripheral blood CD4 +T lymphocytes and CD4 +/CD8 +ratio levels were higher, and the level of CD8 +T lymphocytes was lower after chemotherapy(P<0.05).Conclusion The Ganoderma capsule can significantly down regulate the expression of CD105 protein, VEGF and miR-21 in cervical cancer tumor tissue associated with HPV infection, inhibit the formation of micro blood vessels in tumor tissue, protect the immune function of patients, and improve the clinical curative effect.%目的 探讨灵芝胶囊对伴(human papillomavirus,HPV)感染宫颈癌患者肿瘤组织CD105蛋白及miR-21表达的影响.方法 选取慈溪市中医医院肿瘤科收治的HPV阳性的宫颈癌患者患者162例,按随机数字表法分组,对照组81例予以新辅助化疗,研究组81例在对照组基础上予以灵芝胶囊治疗,检查记录2组间肿瘤组织CD105蛋白、血管内皮生长因子(vascular endothelial growth factor,VEGF)表达水平、肿瘤组织miR-21表达水平、微血管形成相关指标、外周血T淋巴细胞水平变化,同时比较临床疗效.结果 对照组总控制率(72.84%)低于研究组总控制率(86.41%)(P<0.05);与对照组比较,研究组治疗后肿瘤组织CD105蛋白、VEGF表达水平较低,化疗后血清CD105蛋白、转化生长因子β1(transforming growth factor-β1,TGF-β1)、VEGF水平较低,治疗后肿瘤组织miR-21表达水平较低,化疗后外周血CD4+T淋巴细胞、CD4+/CD8+比值水平较高,CD8+T淋巴细胞水平较低,具有统计学意义(P<0.05).结论 灵芝胶囊能够下调伴HPV感染宫颈癌肿瘤组织CD105蛋白、VEGF及miR-21表达,抑制肿瘤组织微血管形成,保护患者的免疫功能,临床治疗效果较好.