中药白芍炮制方法、提取工艺与质量评价研究

摘要

目的 探讨中药白芍炮制方法、提取纯化工艺及白芍质量评价分析方法,为临床选择最佳的炮制方法并提高其质量提供参考依据.方法 选择1年生和3年生中药白芍,分别采用生品和熟品进行炮制,以芍药苷作为代表,采用高效液相色谱法测定含量.采用正交试验优化工艺,测定白芍有效成分并分析质量控制方法.结果 1年生生品白芍中芍药苷的含量为(6.23±0.52)%,1年生熟品白芍中芍药苷的含量为(5.74±0.45)%,比较差异有统计学意义(P<0.05);3年生白芍药材中芍药苷含量为(5.90±0.38)%,3年生熟品白芍中芍药苷含量为(4.85±0.45)%,比较差异亦有统计学意义(P<0.05);以70%乙醇提取3次,时间65 min,溶剂量为6倍,芍药苷收膏率高;通过检测方式的归纳,应筛选出多个单体有效成分对白芍进行含量控制,建立多指标质量控制方法.结论 中药白芍炮制方法表明,生品中有效成分的含量较多,以生品为基础建立中药白芍多指标质量控制方法,逐步完善更全面的白芍质量控制模式,对中药白芍药材的提取工艺进行优化,为白芍的可持续利用及工业化生产提供科学参考.%Objective To study the processing method,extraction technology and quality evaluation analysis of Paeonialactiflora Pall, so as to provide reference for selecting the best method and improving its quality .Methods One year and 3 years Chinese herbal medicine Paeonialactiflora Pall was used.The use of raw materials and cooked products for processing methods to paeoniflorinas the representative , and the content was determined by high performance liquid chromatography.With the optimization of orthogonal test, the methods for the determination of effective components of radix paeoniaealba were summarized and the quality control methods were analyzed .Results The content of paeoniflorin in raw paeonialactiflora pall was (6.33 ±0.52)%,and the content of paeoniflorinin cooked Paeonialactiflora Pall was (5.74 ±0.45)%, which was with statistical significance(P <0.05).The content of paeoniflorin in raw paeonialactiflora pall was (5.90 ±0.38)%,and 4.85 ±0.45% in three years cooked paeonialactiflora pall, and the differenceswere with statistical significance(P<0.05).Extracted three times with 70% ethanol, time 65 min, 6 times the amount of solvent,the paeoniflorin high cream rate was highest.By means of induction, several active ingredients should be screened out to control the content of paeonialactiflora pall to publish multiple indicatorsquality control methods .Conclusion The preparation method of Chinese herb paeonialactiflora pall has showed that the active ingredient content is morein raw herb.To establish quality control method of multiple indicators of paeonialactiflora pall based on raw herb,gradually improve the quality of more comprehensive paeonialactiflora pall quality control model.To optimize the extraction of Chinese herbal medicine,provide scientific reference for the sustainable utilization and industrial production of paeonialactiflorapall.