参附注射液对缺氧缺血性脑损伤新生大鼠凋亡诱导因子表达的影响

摘要

目的:研究参附注射液对新生大鼠脑缺氧缺血后脑皮层凋亡诱导因子(AIF)棱转位和AIF mRNA的影响,探讨参附注射液抑制凋亡的机制.方法:新生7d SD大鼠随机分为假手术组(S)、生理盐水对照组(C)、参附治疗组(SF).每组按照观测的时间点不同分为3h、6h、12h、24h、3d、7d 6个亚组.用Rice法制备新生大鼠HIBD模型.检测不同时间点胞核中AIF、AIFmRNA动态变化及脑皮层组织的凋亡细胞.结果:(1)AIF:C组AIF在胞核内的转位从HI后3h开始增加,24h达到高峰后开始逐渐下降.SF组24h、3d、7d胞棱中的AIF表达较C组减少,2组比较差异有统计学意义(P＜0.05)；(2) AIFmRNA:C组从HI后3h开始增加,24h达到高峰后下降,SF组各时间点均较C组减少(P＜0.05).(3)Tund:HI后3h C组和SF组脑皮层凋亡细胞开始增多,24h达到高峰后下降；SF组在HI后24h、3d、7d均明显低于C组(P＜0.05).结论:参附注射液能够通过抑制AIF的棱转位和基因转录来抑制凋亡,从而起到对HIBD新生大鼠的保护作用.%Objective:To investigate the effects of Shenfu injection on the expression of apoptosis-inducing factor(AIF) in the nucleus and AIF mRNA of cerebral cortex of in neonatal rats with hypoxic-ischemic brain damage(HIBD),in order to explore the mechanism of Shenfu injection inhibiting apoptosis.Methods:Seven-day-old Sprague-Dawley rats were randomly divided into three groups:sham operation group (S),control group (C),Shenfu injection treatment group (SF),They were further divided into six subgroups (n =8 per subgroup) according to the time points 3h,6h,12h,24h,3d,7d.Models of postnatal 7-day Sprague Dawley (SD) rats with HIBD were established with Rice method.In each subgroup,to detect the dynamic changes of AIF in nuclear,AIF mRNA expression and apoptosis cells.Results:(1) AIF translocation to nucleus began as early as 3h after HI,peaked at 24h,then gradually decreased after HI.The expression of AIF in group SF was lower than group C from 24h to 7d (P ＜0.05).(2) AIF mRNA in group S had only very low expression; The expression of AIF mRNA in group C began increase 3h after HI insult,peaked 24 h after HI,then gradually decreased; AIF mRNA of group SF was markedly lower than that of group C at any time (P ＜ 0.05).(3) The number of apoptotic cells of group C and group SF began to increase,peaked at 24h and then decreased after HI; the number of apoptotic cells of group SF at 24h,3d,7d were significantly lower than group C (P ＜ 0.05).Conclusions:Shenfu injection can inhibit apoptosis by inhibiting AIF nuclear translocation and gene transcription,and thus play a protective role in HIBD.