分化抑制因子2在先天性肾盂输尿管连接部梗阻患儿肾盂输尿管连接部组织中的表达

摘要

目的 探讨先天性肾盂输尿管连接部梗阻(PUJO)患儿肾盂输尿管连接部组织中分化抑制因子2 (Id2) mRNA的表达情况.方法 收集20例接受肾盂成形术的PUJO患儿的肾盂输尿管连接部组织,10例对照组标本来自肾母细胞瘤患儿无肿瘤转移的肾盂输尿管组织.按照部位不同将PUJO组分为扩张肾盂、狭窄段及狭窄段下正常输尿管;对照组分为肾盂和输尿管.用半定量反转录-PCR检测Id2 mRNA在不同组间表达水平.结果 PUJO组:Id2 mRNA在扩张肾盂、狭窄段输尿管间表达差异无统计学意义(P>0.05),而此2组与狭窄段下正常输尿管比较均有降低(Pa<0.05);对照组:肾盂和输尿管Id2 mRNA表达差异无统计学意义(P>0.05);PUJO组扩张肾盂、狭窄段输尿管中Id2 mRNA的表达与对照组中肾盂、输尿管表达比较均显著降低(Pa<0.05),而PUJO 组狭窄段下正常输尿管Id2 mRNA表达与对照组肾盂和输尿管比较差异均无统计学意义(Pa>0.05).结论 Id2基因可能在PUJO的发病机制中发挥重要作用.%Objective To explore the expression of inhibitor of differentiation 2 ( Id2 ) mRNA in pelviureteric junction tissue in children with congenital pelviureteric junction obstruction(PUJO).Methods Twenty cases with PUJO and 10 cases with Wilms' tumor without invading into pelviureteric junction tissue as control group were collected.PUJO group was categorized into 3 groups: renal pelvis dilation, narrow ureter and normal ureter below narrow ureter according to their locations.Control group was divided into 2 groups:renal pelvis and ureter.Semi - quantitative reverse transcriptase - polymerase chain reaction was used to detect the expression of Id2 mRNA in these tissues.Results PUJO group: no significant difference of the Id2 mRNA expression was observed between renal pelvis dilation and narrow ureter in PUJO group ( P ＞ 0.05 ), and their expressions were lower than that in the normal ureter below narrow ureter in PUJO group ( Pa ＜ 0.05 ).Control group:the expression of Id2 nRNA between the renal pelvis and ureter were similar( P ＞ 0.05 ).PUJO group and control group:the Id2 mRNA expressions in renal pelvis dilation and narrow ureter in PUJO group were lower than that in renal pelvis and ureter in control group (Pa ＜ 0.05 ).But the Id2 mRNA expressions in the normal ureter below narrow ureter in PUJO group were similar to that in renal pelvis or ureter in control group ( Pa ＞ 0.05 ).Conclusion The Id2 gene may play an important role in the pathogenesis of PUJO in children.