全氟辛烷磺酸与蛋白质体系的共振光散射光谱及其分析应用

摘要

The interaction of bovine serum albumin ( BSA) with perfluorooctane sulfonate ( PFOS) was investigated with resonance light scattering (RLS) spectroscopy. A RLS method for the determination of PFOS was established. In pH =4. 1 Britton-Robinson buffer solution, perfluorooctane sulfonate anions reacted with the protonated BSA by electrostatic forces and hydrophobic interactions to form ion-association complexes and resulted in greatly enhanced resonance light scattering signals characterized by a peak at 285. 0 nm. The RLS intensity of system was proportional to the content of PFOS in the range of 0. 2 ~25. 0 μmol/L, and the limit of detection was 20. 0 nmol/L. The optimum reaction conditions and interference of foreign substances were investigated. This RLS method was applied to the determination of PFOS in environmental samples with RSD ≤4.4%.%研究了牛血清白蛋白(BSA)与全氟辛烷磺酸(PFOS)相互作用的共振光散射(RLS)光谱,建立了PFOS的共振光散射分析方法.在pH值为4.1的BR缓冲溶液中,全氟辛烷磺酸根阴离子与质子化的BSA通过静电引力和疏水作用形成离子缔合物,引起共振光散射强度(IRIS)显著增强,最大散射波长位于285.0 nm处,增强的散射信号强度与PFOS浓度在0.2～25.0 μmol/L范围内呈线性关系,据此建立了测定PFOS的光散射分析方法,检出限为20.0 nmol/L.讨论了体系的最佳反应条件及外来物质的干扰,并探讨了反应机理.建立的共振光散射法用于环境水样中PFOS的测定,RSD≤4.4％.