富马酸泰诺福韦双特戊酯的遗传毒性研究

摘要

Objective To evaluate the genotoxicity of tenofovir dipivoxil fiimarate (TDF) and provide the theory basis for its clinical medication. Methods Salmonella reversion test (Ames test), chromosomal aberration test of CHL cells and mouse marrow micronucleus assay were used to investigate the genotoxicity of TDF. Results Various dosages of TDF showed no mutagenic effects on salmonel latyphimurium in the Ames test, no chromosomal aberration effects were observed after CHL cells exposed to various dosages of TDF with or without rat liver supernatant 9 (S9) and TDF did not have inductive effects on formation of polychromatic erythrocyte micronucleus in mouse bone marrow micronucleus test. Conclusion TDF has no genotoxicity based on the Ames test, CHL chromosomal aberration test and micronucleus assay in the experimental conditions.%目的 检测富马酸泰诺福韦双特戊酯(TDF)的遗传毒性,为临床用药提供理论依据.方法 应用鼠伤寒沙门细菌回复突变试验(Ames试验)、体外培养中国仓鼠肺成纤维细胞(CHL)细胞染色体畸变试验和小鼠骨髓微核试验检测该药物的遗传毒性.结果 该药物对鼠伤寒沙门菌无致突变性,对体外培养CHL细胞染色体无致畸变作用,对昆明小鼠无诱发骨髓嗜多染红细胞微核的效应,三个试验结果均呈阴性.结论 TDF不具有遗传毒性.