生长因子对山羊无腔卵泡卵母细胞体外生长的影响

摘要

In the presence of FSH，the hircine non-antral follicle oocytes were cultured for 9 days with IGF-I，EGF and bFGF alone or combinedly．The results showed that IGF-I（100ng／mL）effectively maitained the survival of follicle oocytes and promoted their growth，and the survival rate and the relative growth rate of oocytes were 75.00% and 45.06% respectively．EGF（50ng／mL）enhanced the survival rate of the oocytes like IGF-I，but had a negative effect on the growth of oocytes．bFGF（50ng／mL）had a promoting effect on the survival in vitro of oocytes but not obvious on the growth of them （the survival rate and the relative growth rate of oocytes were 76.92% and 31.55% respectively）．IGF-I（100ng／mL） and EGF（50ng／mL）combination had the highest effects on both of the survival and the growth of oocytes．The survival rate and the relative growth rate of oocytes were 90.91% and 48.24% respectively．It has been shown that in the presence of FSH these kinds of growth factors alone or choosingly combinedly gave a positive regulation to the survival in vitro of hircine non-antral follicle oocytes．%山羊无腔卵泡卵母细胞在胰岛素样生长因子I（IGF-I）和表皮生长因子（EGF）及碱性成纤维细胞生长因子（bFGF）单独或联合存在时，与FSH一道体外培养9d，结果表明，IGF-I（100ng／mL）能有效地维持卵泡卵母细胞的存活并促进其生长，存活率达75.00%，相对生长率达45.06%；EGF（50ng／mL）能提高卵泡卵母细胞的体外存活率，类似于IGF-I，但对其生长相对表现出抑制影响；bFGF（50ng／mL）对卵泡卵母细胞的体外存活具有促进作用，但对卵母细胞生长的促进作用不明显（卵母细胞的存活率为76.92%，相对生长率为31.55%）。IGF-I（100ng／mL）和EGF（50ng／mL）联合存在时，不论是对卵泡卵母细胞的存活，还是对生长均产生了最好的效果，卵母细胞存活率达到90.91%，相对生长达48.24%。本试验结果一个共同点是在FSH存在的情况下，这3种生长因子单独或有选择的联合，对山羊卵泡卵母细胞的体外存活能力均具有正向调节作用。