不同来源禽网状内皮增生病病毒株的致病性比较

摘要

This experiment was conducted to investigate the pathogenicity of different Reticuloen-dotheliosis virus (REV) strains on 1-day-old SPF chickens. 1-day-old SPF chickens were inocula-ted with six REV strains including REV-C99, a cloned strain. The pathogenicity of REV from different strains was evaluated by the measurement of growing rate of chicks and the antibody ti-ters to the Newcastle disease virus(NDV) and Avian influenza virus (H9-AIV and H5-AIV) after vaccination. The results showed that REV challenge at a dose of 3 000 TCID_(50)/bird resulted in the significantly (P<0.05) suppressed HI titers of NDV, AIV(H5-, H9-) at the 4-, 5- and 6-week after vaccination compared to control group, whereas there was no significant difference be-tween the 6 REV strains. The results indicated that REV challenge at 1-day of age would retard the growth of chicks and suppressed the humoral immunity. The result suggests that REV chal-lenge is one of the important reasons of immune failure.%本研究旨在探讨不同来源的禽网状内皮增生病病毒(REV)对1日龄SPF鸡的致病性.用包括分子克隆化病毒REV-C99在内的不同来源的6个REV株人工感染1日龄SPF鸡,以禽流感病毒灭活疫苗(H9-AIV、H5-AIV)与新城疫病毒灭活疫苗(NDV)免疫后HI抗体滴度的测定结果为指标,比较不同REV株的致病性.结果表明,分别用3000TCID_(50)·只~(-1)的剂量人工感染1日龄SPF鸡,6个REV感染组与对照组相比,H9-AIV、H5-AIV与NDV免疫后4、5与6周的HI抗体水平均极显著降低(P<0.01).但6个REV感染组之间差异不显著(P>0.05).研究结果显示,不同来源REV株感染1日龄SPF鸡后均造成生长迟缓,并对体液免疫反应有明显的抑制作用,同时,这也揭示出REV感染可能是免疫失败的重要原因之一.