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SCAR分子标记对水貂食毛症遗传病因的初步研究

     

摘要

In order to discuss the causes of mink trichophagia at molecular level, SCAR(sequenced characterized amplified region) technology was used to analyze genetic constitution of the healthy and trichophagia mink groups. Four polymorphic primers were screened from 126 random primers by RAPD. The different bands were amplified in healthy and sick mink groups. The specific bands were then cloned and sequenced, two SCAR primers were designed according to their sequence information by primer A20 and S139. Then PCR amplification was carried out in the healthy and trichophagia mink groups. The results showed that the frequency of SA20-757 in the two groups was 86. 88% and 27. 41%, respectively, and x2 test of polymorphic fragments in the two groups showed significant difference (P

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