普氏野马与两种新疆地方品种马的ELA-DRA*exon2的多态性分析

摘要

To understand the polymorphism and allele frequency distribution of ELA-DRA * exon2 in Przewalski,s horse(Equus PrzewaLskii) , Kazakh and Yanqi horse, ELA-DRA * exon2 in three types of horses was genotyped by PCR-SSCP methods. Both the nucleotide and the amino acid sequences of the different alleles were analyzed in these horses. The results showed that seven genotypes were detected in 127 horses: three homozygotes named AA, BB and CC, four heterozygotes named AB, BC, AC and AD. AA genotype was dominant in Kazakh and Yanqi horses.CC was the dominant genotype in Przewalski's horse(Equus Przewalskii). The chi-square analysis suggested that the allele and genotype frequencies were in Hardy-Weinberg equilibrium in the three horse types. The analysis of PIC and He showed that the three horse types belonged to intermediate polymorphism. The values of PIC and He in Przewalski's horse(Equus Przewalskii)were lower than that in Kazakh and Yanqi horses.%为了解普氏野马、哈萨克马与焉耆马的ELA-DRA*exon2的多态性以及等位基因频率分布.本研究采用PCR-SSCP技术对普氏野马、哈萨克马与焉耆马的ELA-DRA*exon2进行分型,并对不同等位基因进行核苷酸与氨基酸分析.结果显示:127匹马中共出现7种基因型,3种纯合子分别记为AA、BB、CC;4种杂合子分别记为AB、AC、BC、AD.AA基因型为哈萨克马与焉耆马的优势基因型,普氏野马以CC基因型为主.经x2检验后,3种类型马的等位基因频率和基因型频率均处于Hardy-Weinberg平衡状态.PIC值与He值分析表明,3种类型马均属于中度多态,且普氏野马的PIC值与He值比哈萨克马与焉耆马低.