本研究以湖羊肝脏为材料对肝受体类似物-1(Liver receptor homolog-1,Lrh-1;NR5A2)基因序列进行RT-PCR和RACE测定,并用DNAman、Tmpred、Signal P 3.0、Expasy等生物信息学分析软件和在线工具,对Lrh-1 cDNA序列及其蛋白的理化特性、跨膜结构、信号肽和二级结构进行生物信息学分析,同时采用实时荧光定量PCR方法检测湖羊Lrh-1基因的组织表达谱.结果表明,湖羊Lrh-1基因cDNA序列全长1 488 bp,与牛的核酸序列相似度最高,为98％,编码区共编码495个氨基酸,氨基酸序列与牛、人、马、猴、犬、小鼠、褐鼠的氨基酸同源性分别为98％、97％、96％、97％、97％、86％和86％；Lrh-1 mRNA在湖羊脑、消化及生殖系统组织中均有表达且具有组织特异性,其中在下丘脑组织中的表达量相对最高.%In the study, the full-length cDNA of the liver receptor homolog-1 (Lrh-l) was detected from liver of Hu sheep by reverse transcription PCR and RACE methods. Lrh-l cDNA sequence, protein physicochemical properties, membrane structure, signal peptide and secondary structural characterisitics were analyzed and predicted with bioinformatics softwares and online tools ,such as DNAman, Tmpred, SignalP 3. 0, TargetP 1. 1, Expasy, and PSORT II prediction. The expression of Lrh-l mRNA in different tissues of Hu sheep was also examined by quantitative real-time PCR. The results showed that the cDNA of Hu sheep Lrh-l contained 1 488 nu-cleotides, and the nucleotide sequence had 98% homology with that of Bos taurus. It encoded 495 amino acid residues, which shared 98%, 97%, 96%, 97%, 97%, 86% and 86% identity with those of Bos taurus , Homo sapiens , Equus caballus , Macaca mulatta , Canis familiaris , Mus musculus and Rattus norvegicus , respectively. The relative expression level of Lrh-l mRNA was detected in brain, digestive system and reproductive system, and the expression levels were showed differently and had tissue-specific. Relatively, Lrh-l mRNA expression level was the highest in hypothalamus of Hu sheep.
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