牛传染性鼻气管炎病毒内蒙古分离株gB、gC、gD基因的分子克隆与序列分析

摘要

对牛传染性鼻气管炎病毒(IBRV)内蒙古分离株gB、gC、gD基因进行克隆和序列测定,结果表明IBRV内蒙古分离株gB、gC、gD基因序列分别为2 850、1 723、1 559 bp,编码933、508、417个氨基酸组成的完整开放阅读框.序列比较和系统进化树分析结果显示:内蒙古分离株与其他毒株gB、gc、gD基因核苷酸序列的相似性为94.9％～99.9％,其推导的氨基酸相似性在90.3％～99.7％,不同IBRV毒株gB、gC、gD基因在核苷酸和氨基酸水平上高度保守.在系统进化树中内蒙古分离株与瑞典毒株亲缘关系较近,属于同一个分支.%The gB, gC and gD genes of Inner Mongolia IBRV strain were cloned and sequenced. The results showed that the gB, gC and gD genes were 2 850, 1 723 and 1 559 bp, respectively. And the 3 genes contained their corresponding open reading frames (ORF) which encoding 933, 508 and 417 amino acids, respectively. The gB, gC and gD genes of Inner Mongolia strain shared 94.9% to 99. 9% homology in nucleotide with others strain from GenBank, and shared 90. 3% to 99.7% homology in deduced amino acid sequence by the sequences and phylogenetic analysis. The gB, gC and gD genes were highly conserved among IBKV strains. Phylogenetic tree of IBRV revealed that the Inner Mongolia strain was consistently located in the same lineages with Sweden strain.