FSHR基因第1外显子多态性及其与小梅山猪产仔数的关系

摘要

The present study was to detect the polymorphism of exonl of follicle-stimulating hormone receptor (FSHR) gene and investigate the relationship between FSHR gene and litter size in pigs. The polymorphism of exonl of FSHR gene was investigated by PCR-SSCP and direct sequencing methods and the effect of exonl of FSHR gene on litter size in Xiaomeishan pigs was analyzed using the least square analysis, simultaneously the variance components were analyzed and the selective reaction was predicted. The results showed that three SNPs (C70T, C74G and C81T) were detected in exonl of FSHR gene, of which C74G caused amino acid change. Simultaneously three haplotypes and five genotypes were screened out. The difference of FSHR gene haplotypes and genotypes distribution among Xiaomeishan pigs, Fengjing pigs and Large White pigs reached extremely significant level (P<0. 01). According to the result of correlation analysis, in Xiaomeishan pigs, the TNB of the sows with genotype A A and AC after the second parity were 0. 64 (P>0. 05) and 0. 36 (P>0. 05) piglets less than those with genotype BC, and the TNB and NBA of the sows with genotype AA in all parities were 0. 80 (P<0. 01) and 0. 67 (P< 0. 01) piglets less than those with genotype AC. The heredity of TNB and NBA in Xiaomeishan pigs was mostly influenced by the genetic dominant effect in exonl of FSHR gene.%本研究旨在检测猪FSHR基因第1外显子多态性,并分析其与产仔数之间的关系.采用PCR- SSCP和直接测序方法检测基因多态性,利用最小二乘法分析其与母猪产仔数的关系,同时对多态位点的方差组分进行分析,并预测选择反应.研究结果表明:猪FSHR基因第1外显子区域内检测到3个SNPs(C70T、C74G、C81T),其中C74G导致氨基酸的改变,同时,筛选出3个单倍型和5种基因型；FSHR基因单倍型与基因型分布在小梅山猪、枫泾猪与大白猪群体间差异达到极显著水平(P＜0.01);关联分析结果显示,2胎以上的小梅山母猪中,AA和CC基因型个体的TNB比BC基因型分别低0.64(P＞0.05)和0.36头(P＞0.05),所有胎次中,AA基因型个体的TNB 和NBA比AC基因型分别低0.80(P＜0.01)和0.67头(P＜0.01).对于FSHR基因第1外显子而言,小梅山猪TNB和NBA的遗传主要受到显性效应的影响.