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Primer set for detecting EGFR exon 21L858R gene polymorphism and use thereof
Primer set for detecting EGFR exon 21L858R gene polymorphism and use thereof
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机译:用于检测EGFR外显子21L858R基因多态性的引物组及其用途
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摘要
The invention provides a primer set for detecting a polymorphism in EGFR exon 21 1 L858R. The primer set has a P1 oligonucleotide and a P2 oligonucleotide and can perform amplification by using a region including the 172792nd base of SEQ ID NO: 1 as a template. As a base that is complementary to the 172792nd base of SEQ ID NO: 1, the P1 oligonucleotide has cytosine and the P2 oligonucleotide has adenine. The melting temperature of the P1 oligonucleotide is higher than the melting temperature of the P2 oligonucleotide, and/or the P1 oligonucleotide is one or more bases longer than the P2 oligonucleotide. The invention further provides a polymorphism detection primer, a polymorphism detection method using the primer set, a method of evaluating a EGFR tyrosine kinase inhibitor using the primer set, a primer used in the polymorphism detection method, and a kit including the primer set.
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机译:本发明提供了用于检测EGFR外显子21 1 L858R中的多态性的引物组。引物组具有P1寡核苷酸和P2寡核苷酸,并且可以通过使用包括SEQ ID NO:1的第172792位碱基的区域作为模板来进行扩增。作为与SEQ ID NO:1的172792位碱基互补的碱基,P1寡核苷酸具有胞嘧啶,而P2寡核苷酸具有腺嘌呤。 P1寡核苷酸的解链温度高于P2寡核苷酸的解链温度,和/或P1寡核苷酸比P2寡核苷酸长一个或多个碱基。本发明进一步提供了一种多态性检测引物,使用该引物对的多态性检测方法,使用该引物对评估酪氨酸激酶抑制剂的方法,用于该多态性检测方法的引物以及包括该引物对的试剂盒。
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