16S rRNA高通量测序方法检测羊圈空气微生物群落结构及多样性

摘要

The aim of this study was to investigate the bacterial community structure and the characteristics of pathogenic bacteria in the air of sheepfold.We selected ten different sheep farms to take air samples.The Illumina MiSeq sequencing with 16S rRNA V3-V4 variable region was adopted to analyze the bacterial community structures of the air in sheepfold.A total number of 33 408 operational taxonomic units (OTUs) were obtained from air samples under the similarity level of 97％.We have got bacteria comprising groups of 25 phyla,66 classes,123 orders,253 families,547 genera,and 444 species.Analysis of microbial diversity and summarize pathogens with higher abundance in sheepfolds,which provides the basis for the disinfection and medication for sheep farm.The diversity,abundance,and characteristics of the main pathogens in air samples were analyzed in this study,which would be a helpful reference for disease prevention and control in sheepfold.%以羊圈空气微生物为研究对象,了解羊圈空气中细菌群落结构以及致病菌属特征.将来自10个不同羊场的羊圈空气样本,应用Illumina MiSeq高通量测序技术测定羊圈空气中细菌的16S rRNAV3-V4变异区序列,分析不同空气样本细菌群落组成.结果显示:空气样本中的细菌在97％的相似水平下共得到OTU个数为33 408,涵盖了25门、66纲、123目、253科、547属、444种的细菌.通过微生物多样性进行分析以及羊圈空气样本菌群中丰度较高的病原菌属的统计,以期为羊场的消毒和用药提供依据.本研究解析了羊圈空气样本中微生物的多样性、丰度及主要病原菌属的特征,对羊场疾病防控有一定指导意义.