2014-2015年豫南地区猪流行性腹泻病毒S基因变异分析

摘要

为分析河南地区猪流行性腹泻病毒(PEDV)的变异情况,本研究设计合成扩增S基因全长的引物,利用RT-PCR方法,对2014-2015年采集病料中38株PEDV S基因进行扩增、克隆和序列测定,并根据结果构建进化树和进行序列比对分析.S基因进化树分析表明:所有PEDV S基因扩增片段与近年来中国和美国分离的变异毒株亲缘关系较近.序列比对分析表明:扩增的PEDV S基因编码的S蛋白中存在氨基酸的插入和缺失.并且,一些扩增S基因片段在编码的S蛋白的C端有11个氨基酸的缺失.许多扩增的S基因在编码的S蛋白的中和表位COE和2C10区域存在氨基酸突变.这些结果为了解河南地区PEDV变异流行情况和控制PED的暴发提供了基础研究理论.%To investigate the variation of PEDV S gene in Henan province,specific primers were designed and synthesized,S genes of 38 PEDV strains from different pig farms in Henan provinces from 2014-2015 were amplified by RT-PCR,then cloned and sequenced.The phylogenetic relationships and protein characterization of the full-length PEDV S protein in Henan of China were analyzed with other PEDV reference strains.Phylogenetic analysis indicated that all the amplified S genes had a close relationship with some variant strains isolated from China and USA in recent years.Sequence alignment analysis showed that extensive amino acid (AA) insertion and deletion were found in the S protein.Furthermore,there were 11 AA deletion at the C terminal of S protein encoded by amplicated S gene.Many strains had wide AA substitution in the neutralizing epitopes COE and 2C10.These results will provide valuable research basis for understanding the variant characterization of PEDV and controlling the outbreak of PED in this area.