首页> 中文期刊>畜牧兽医学报 >川藏地区牦牛牛病毒性腹泻病毒分子流行病学调查及分离鉴定

川藏地区牦牛牛病毒性腹泻病毒分子流行病学调查及分离鉴定

     

摘要

为了解川藏高原地区牦牛牛病毒性腹泻病毒(bovine viral diarrhea virus,BVDV)的感染情况和牦牛BVDV的主要流行亚型及遗传变异情况,采用RT-PCR方法,对四川和西藏部分高原地区采集的149份临床腹泻牦牛粪便开展分子流行病学调查,根据5'-UTR、Npro和E2基因的变异研究该地区牦牛BVDV的遗传变异情况.同时,通过RT-PCR、病毒分离培养和间接免疫荧光检测等方法鉴定牦牛BVDV分离株.结果表明,149份粪便中,BVDV阳性检出率为19.46%[95%置信区间(CI)=13.4%~26.7%],其中西藏地区牦牛BVDV阳性检出率为27.14%(95%CI=17.2%~39.1%),四川地区牦牛BVDV阳性检出率为12.66%(95%CI=6.2%~22.0%).随机抽取10份阳性样本测序,根据5'-UTR、Npro和E2基因序列建立系统发育进化树分析,10份样本为BVDV-1型,包括BVDV-1a(n=4)和1d(n=6)亚型.此外,成功分离鉴定出两株致细胞病变型(cytopathic,cp)BVDV,分别属于牦牛BVDV-1a和1d亚型,命名为SWU-Z10和SWU-L8.研究结果提示,川藏部分高原地区牦牛存在BVDV感染,BVDV-1a和1d为牦牛感染的主要亚型.本研究丰富了BVDV的分子流行病学资料,也为后续研制牦牛专用BVDV疫苗提供理论基础.%This study was conducted to investigate the infection status of Bovine viral diarrhea virus (BVDV) and the main epidemic subtypes and genetic variation of BVDV in the Sichuan-Tibet Plateau region.A total of 149 clinical diarrhea yak fecal samples were collected from Sichuan province and Tibet region in 2016,and the genetic variation of BVDV in yak was studied according to the variation of 5'-UTR,Npro and E2.Meanwhile,the BVDV strain of yak was isolated and identified by RT-PCR,cell isolation and indirect immunofluorescence.The results showed that the positive rate of BVDV was 19.46% (95% confidence interval (CI)=13.4%-26.7%) in 149 samples of clinical diarrhea yak feces.The positive rate of BVDV in yak in Tibet region was 27.14% (95% CI=17.2%-39.1%) and 12.66% (95% CI=6.2%-22.0%) in Sichuan province.The phylogenetic tree was established based on the sequences of 5CUTR,Npro and E2.The results showed that 10 samples belongs to BVDV-1,BVDV-1a subtype (n=4) and 1d (n=6).In addition,two strains of cytopathic (cp) BVDV were identified and identified as subgroups of BVDV-1a and 1d,named SWU-Z10 and SWU-L8,respectively.The results showed that BVDV infection is present in yak in the Sichuan-Tibet Plateau region,and BVDV-1a and 1d are the dominant subtype of yak infection in Sichuan-Tibet Plateau region.The results of the study provide basic data for the comprehensive prevention and control of yak diarrhea in Sichuan-Tibet Plateau region.The data obtained enrich the molecular epidemiological investigation data of BVDV,and provide the theoretical basis for the development of BVDV vaccine for yak.

著录项

  • 来源
    《畜牧兽医学报》|2018年第3期|606-613|共8页
  • 作者单位

    西南民族大学生命科学与技术学院,成都610041;

    西南民族大学生命科学与技术学院,成都610041;

    四川省龙日种畜场,红原624400;

    西南民族大学生命科学与技术学院,成都610041;

    西南民族大学生命科学与技术学院,成都610041;

    西南民族大学生命科学与技术学院,成都610041;

    国家民族事务委员会青藏高原动物疫病防控创新团队,成都610041;

    西南民族大学生命科学与技术学院,成都610041;

    国家民族事务委员会青藏高原动物疫病防控创新团队,成都610041;

    西南民族大学生命科学与技术学院,成都610041;

    国家民族事务委员会青藏高原动物疫病防控创新团队,成都610041;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 S852.659.6;
  • 关键词

    BVDV; 川藏地区; 牦牛; 分子流行病学调查; 病毒分离培养;

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