本试验旨在采用实验室建立的肉仔鸡体外消化模型对玉米-豆粕型饲粮中添加不同水平β-甘露聚糖酶的作用效果进行评定.分别在体内和体外条件下,测定了添加0、300、600、1 200、2400 U/kgβ-甘露聚糖酶的肉仔鸡饲粮干物质、粗蛋白质、能量的消化率和肉仔鸡生产性能,并进行了相关性分析.肉仔鸡饲养试验结果表明,与不添加β-甘露聚糖酶的对照组相比,在肉仔鸡玉米-豆粕型饲粮中添加β-甘露聚糖酶可以提高干物质、粗蛋白质和能量的消化率,其中以β-甘露聚糖酶添加量为600 U/kg组效果最好;体外消化试验结果与肉仔鸡饲养试验结果趋势一致;相关性分析结果表明,添加β-甘露聚糖酶后,干物质、粗蛋白质和能量的体外消化率与肉仔鸡平均日增重均存在相关性(R2=0.792 3、0.860 9、0.773 0),与养分回肠表观消化率测定值之间存在强相关(R2=0.989 9、0.907 3、0.985 2).利用建立的肉仔鸡体外消化模型可以评定玉米-豆粕型饲粮中添加β-甘露聚糖酶对肉仔鸡生产性能和营养物质消化率的影响.%This experiment was conducted to investigate the effects of different supplemental levels of β-mannanase on broilers fed corn-soybean meal basal diet based on an in vitro digestion method. The digestibility of dry matter ( DM) , crude protein ( CP) and energy of the diets supplemented with β-mannanase at 0, 300,600, 1200 and 2400 U/kg was determined in vivo and in vitro, and the performance of the broilers was also determined. A correlation analysis was carried out additionally. The results of the in vivo experiment showed that compared with the control group, β-mannanase added into the basal diets at the levels of 300 to 2400 U/kg could increase the digestibility of DM, CP and energy, and the highest digestibility of nutrients was achieved at the supplemental level of 600 U/kg. The results of the in vitro experiment had the same trend with the in vivo one. The results of the correlation analysis showed that the in vitro digestibility of DM, CP and energy had correlations with the the average daily gain of the broilers ( R2 =0. 792 3, 0. 860 9, 0. 773 0) , and had a strong correlation with the ileal apparent digestibility of nutrients in broilers ( R2 = 0. 989 9, 0. 907 3, 0. 985 2 ) .These results indicate that the in vitro digestion method is suitable to evaluate the effects of β-mannanase supplemented to the corn-soybean meal diets on the performance and nutrient digestibility of broilers. [ Chinese Journal of Animal Nutrition ,2011 ,23 ( 1 ) : 130-135 ]
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