饲粮蛋白质源对断奶仔猪小肠全基因组转录谱的影响

摘要

To explore molecular mechanism of dietary protein sources affecting development of intestinal tract of weaner piglets, porcine genome array (PGA) was used to detect intestinal transcription profile of weaner piglets (0, 3, 7 and 14 d after weaning) fed diets with different protein sources. Twenty-four male piglets were randomly divided into two treatments. Piglets in treatment 1 were fed the diet, 30% total protein of which were substituted by milk protein, and those in treatment 2 were fed a diet with whole phytoprotein sources. The results showed as follows; 1) there were 370 differentially expressed genes in treatment 1 (P<0.05). Series test clustering (STC) analysis revealed 26 expression profiles, in which there were 3 significant expression profiles, and significance levels of profiles 5 and 11 (P <0. 01) were higher than those of the other profiles. Meanwhile, there were 263 differentially expressed genes in treatment 2 (P <0. 05). Twenty-six expression profiles were obtained by STC analysis, in which significance levels of profiles 3 and 22 (P <0. 01) were higher than those of the other profiles. 2) Gene co-expression network analysis revealed the molecular regulation mechanism of dietary protein sources affecting intestinal tract development of weaner piglets. A total of 12 and 9 valuable important candidate genes in regulatory network were successfully screened from the piglets in 2 treatments by PGA analysis, and 9 co-expressed genes were also obtained, and their regulation in network were affected by dietary treatment. In conclusion, some potential genes associated with intestinal tract development and function affected by dietary protein sources in weaner piglets are screened, and these candidate genes may deserve further investigation.%为探讨饲粮蛋白质源影响断奶仔猪小肠发育的分子机制,本试验利用猪全基因组表达谱芯片分析不同蛋白质源饲粮下断奶仔猪(断奶0、3、7和14 d)小肠转录谱,24头仔公猪随机分为2个处理,处理1采用乳源蛋白质30％替代饲粮总蛋白质,处理2采用全植物蛋白质源饲粮.结果表明:1)处理1获得370个差异表达基因(P＜0.05),采用序列试验聚类分析,共获得26个表达模式,其中有3个显著表达模式,模式5和11显著性水平最高(P＜0.01).处理2获得263个差异表达基因(P＜0.05),序列试验聚类分析得到26个表达模式,其中模式3和22显著水平最高(P＜0.01).2)基因共表达网络构建和分析一定程度上揭示了不同蛋白质源饲粮对肠道发育的分子调控机制,以及两者之间的差异.通过基因芯片数据,从2种蛋白质源饲粮处理下的基因调控网络中筛选出12和9个具有重要影响和研究价值的基因,以及9个在2个处理中网络调控地位发生改变的共表达基因.通过基因芯片数据,从2种蛋白质源饲粮处理下的基因调控网络中筛选出12和9个具有重要影响和研究价值的基因,以及9个在2个处理中网络调控地位发生改变的共表达基因.总之,本试验筛选出了一些在不同饲粮蛋白质源影响断奶仔猪肠道发育和功能过程中可能具有深入研究意义的候选基因.