海兰褐壳蛋鸡含黄素单氧化酶3基因型频率分布及其对蛋品质的影响

摘要

This experiment was conducted to investigate the distribution of T329 S mutation in flavin-containing monooxygenase 3 ( FMO3 ) in Hy-Line brown laying hens, and the effects of FMO3 genotype on egg quality and egg trimethylamine ( TMA ) content. A polymerase chain reaction-restriction fragment length polymor-phism ( PCR-RFLP) followed by DNA sequencing assay was developed to genotype laying hens. A total of 120 genotyped laying hens were selected and fed for 6 weeks, and the egg quality and egg TMA contents were analyzed. The results showed as follows:1) the frequencies of AA (wild type), AT (heterozygous type) and TT (mutant type) genotype were 3. 4%, 76. 6% and 20. 0%, respectively. The allelic frequencies were not in Hardy-Weinberg equilibrium in the Hy-Line brown laying hens strain ( P<0 . 001 ) . 2 ) Egg quality was not affected by the genotype ( P>0 . 05 ) with the exception of TMA contents which was higher in TT hens ( P<0 . 05 ) . However, yolk TMA content remained below the human detection threshold for TT hens. The results indicate that the frequency of TT hens in Hy-Line brown strain is 20 . 0%, which show higher egg TMA con-tent. Egg quality is not affected by the genotype.%本试验旨在检测含黄素单氧化酶3( FMO3) T329 S突变位点在我国商品代海兰褐壳蛋鸡群体中的分布，考察基因型对鸡蛋品质和鸡蛋中三甲胺( TMA )含量的影响。采用聚合酶链式反应-限制性片段长度多态性分析方法( PCR-RFLP)和DNA测序技术测定FMO3 T329S突变位点在商品代海兰褐壳蛋鸡群体中的分布。筛选已知FMO3基因型海兰褐壳蛋鸡120只，饲养6周，测定鸡蛋品质和鸡蛋中TMA含量。结果表明：1)商品代海兰褐壳产蛋鸡群体中， AA (野生型)、AT(杂合型)和TT(突变型)基因型频率分别为3.4%、76.6%和20.0%，极显著偏离了哈代-温伯格平衡( P<0.001)。2)基因型显著影响鸡蛋TMA含量，但对鸡蛋其他品质无显著影响(P>0.05)；TT基因型蛋黄TMA含量显著升高(P<0.05)，但低于嗅觉阈值。由此可见，海兰褐壳鸡群体中TT基因型出现频率较高(20.0%)，其蛋中TMA含量较高；FMO3基因型不影响蛋品质。