地顶孢霉培养物对大鼠生长性能、血清与肝脏抗氧化及免疫指标的影响

摘要

本试验旨在研究地顶孢霉培养物对大鼠生长性能、血清与肝脏抗氧化以及免疫指标的影响。选用体重相近的SD大鼠40只，随机分成5组，每组8个重复，每个重复1只大鼠。5组分别是对照组和4个不同地顶孢霉培养物剂量组（10、50、250和1250 mg／kg BW组），连续灌胃21 d。结果表明：地顶孢霉培养物极显著提高大鼠的平均日增重（ P＜0．01）；提高了大鼠肝脏指数、胸腺指数；提高了大鼠血清中总蛋白（ TP ）、白蛋白（ ALB ）、球蛋白（ GLB ）和葡萄糖（ GLU）含量，降低了谷丙转氨酶（ ALT）、谷草转氨酶（ AST）活力和低密度脂蛋白（ LDL）含量；提高了血清和肝脏中谷胱甘肽过氧化物酶（ GSH⁃Px）活力和总抗氧化能力（ T⁃AOC），降低丙二醛（ MDA）含量，同时提高了肝脏中的总超氧化物歧化酶（ T⁃SOD）含量；提高了血清免疫球蛋白A、免疫球蛋白G和免疫球蛋白M含量，同时提高血清和肝脏中白介素－4（ IL⁃4）含量，降低白介素－1β（ IL⁃1β）和白介素－17（ IL⁃17）含量。综合各项指标，地顶孢霉培养物能够提高SD大鼠平均日增重，改善血清生化指标，提高大鼠机体抗氧化能力和免疫能力，其中以250 mg／kg BW的添加量为最佳。%This study was aimed to investigate the effects of Acremonium terricola culture on growth perform⁃ance, antioxidant and immune indexes of serum and liver in Sprague Dawley rats. Fourty Sprague Dawley rats with near weight were randomly divided into 5 groups with 8 replicates per group and 1 rat per replicate. One was control group and others were experimental groups with different doses of Acremonium terricola culture (10, 50, 250 and 1 250 mg/kg BW, respectively). Acremonium terricola culture was given corresponding medicines for 21 days via gavage. The results showed as follows: Acremonium terricola culture significantly improved average daily gain of rats ( P<0.01);improved liver and thymus indexes;improved serum total pro⁃tein ( TP) , albumin ( ALB) , globulin ( GLB) and glucose ( GLU) contents, decreased serum activities of al⁃anine transaminase (ALT) and aspertate transaminase (AST), and serum content of low⁃density lipoprotein (LDL); improved glutathione peroxidase (GSH⁃Px) activity and total antioxidant capacity (T⁃AOC), de⁃creased malondialdehyde ( MDA) content of serum and liver, and improved superoxide dismutase ( T⁃SOD) activity of liver; increased serum contents of immunoglobulins A, M and G, increased interleukin⁃4 ( IL⁃4) content of serum and liver, and decreased contents of interleukin⁃1β ( IL⁃1β) and interleukin⁃17 ( IL⁃17 ) of serum and liver. In conclusion, Acremonium terricola culture can improve average daily gain, biochemical in⁃dexes, antioxidant and immunity of serum and liver in Sprague Dawley rats, and the optimal supplemental lev⁃el is 250 mg/kg body weight.