海兰褐蛋鸡体内Toll样受体的分子克隆与序列分析

摘要

To study the innate immunity of the Hyline Brown hens, 14 pairs of specific primers were designed to specifically amplify 9 Toll-like receptor (TLR) genes of chickens and 3 Toll-like 9 receptor gene of human, mouse and pig. A total of 11 chicken TLR genes were amplified and compared with those available in the GenBank. The homology percentage of chicken TLRs was more than 99%. However, TLR21 was present in chickens but TLR9 was absent.%　　为了研究海兰褐蛋鸡的先天性免疫机制，根据已报道的鸡的9种Toll样受体（TLR）和其他动物（人、小鼠和猪） TLR9的基因序列，设计特异性引物，从海兰褐蛋鸡体内扩增出11个鸡Toll样受体部分基因片段，与GenBank登录的序列进行比对和分析，同源率达99%以上。同时也证实鸡体内不存在TLR9，但是存在TLR21。