JNK信号通路在紫杉醇诱发大鼠海马神经元凋亡中的作用:与NF-κB通路的关系

摘要

Objective To evaluate the role of c-Jun N-terminal kinase (JNK) signaling pathway in paclitaxel-induced apoptosis in hippocampal neurons of rats, and the relationship with nuclear factor kappa B (NF-κB) pathway.Methods The primarily cultured hippocampal neurons were seeded in 96-well plate at a density of 1×106 cells/ml (200 μl/hole) , and were randomly divided into 4 groups (n=8 each) using a random number table: control group (C group), paclitaxel group (P group), JNK inhibitor SP600125 group (S group), and SP600125 + paclitaxel group (S+P group).Paclitaxel 2 ml (1 μmol/L) was added to group P.SP600125 2 ml (10 μmol/L) was added to group S.In group S+P, SP600125 2 ml (10 μmol/L) was added, the cell were then incubated for 1 h, and then paclitaxel 2 ml (1 μmol/L) was added.The cells were then incubated for 24 h.At 24 h of incubation, the apoptosis in hippocampal neurons was detected by flow cytometry, and the expression of NF-κB p65 was measured by Western blot.The apoptosis rate was calculated.Results Compared with group C, the apoptosis rate was significantly increased, and the expression of NF-κB p65 was up-regulated in P and S+P groups, and the apoptosis rate was significantly decreased, and the expression of NF-κB p65 was down-regulated in group S (P＜0.05).Compared with group P, the apoptosis rate was significantly decreased, and the expression of NF-κB p65 was down-regulated in group S+P (P＜0.05).Conclusion JNK signaling pathway mediates paclitaxel-induced apoptosis in hippocampal neurons of rats, and the mechanism is likely related to inhibition of NF-κB pathway activation.%目的 评价c-Jun氨基末端激酶(JNK)信号通路在紫杉醇诱发大鼠海马神经元凋亡中的作用及其与NF-κB通路的关系.方法 培养大鼠原代海马神经元,以1×106个/ml密度接种于96孔培养板,200 μl/孔,32个培养孔,采用随机数字表法,将其分为4组(n=8):正常对照组(C组)、紫杉醇组(P组)、JNK抑制剂SP600125组(S组)和SP600125+紫杉醇组(S+P组).P组加入2 ml紫杉醇(1 μmol/L);S组加入2 ml SP600125(10 μmol/L);S+P组先加入2 ml SP600125(10μ ,mol/L),孵育1h时加入2 ml紫杉醇(1μmol/L).紫杉醇孵育24 h时,采用流式细胞术测定海马神经元凋亡率,采用Western blot法测定NF-κB p65表达.结果 与C组比较,P组和S+P组神经元凋亡率升高,NF-κB p65表达上调,S组神经元凋亡率降低,NF-κB p65表达下调(P＜0.05);与P组比较,S+P组神经元凋亡率降低,NF-κB p65表达下调(P＜0.05).结论 JNK信号通路介导了紫杉醇诱发大鼠神经元凋亡的过程,其机制可能与抑制NF-κB通路激活有关.