Objective To investigate the effects of glial cell derived neurotrophic factor on the levels of stem cell factor (SCF) and SOD CAT MDA in testis tissue of mice with cryptorchidism.Methods Total of 48 male mice model with left cryptorchidism were randomly divided into four groups(12 in each group), including Group A(cryptorchidism group), Group B (cryptorchidism + NS), Group C (cryptorchidism + GDNF), Group D(the normal control group).Mice in different group were treated by different processing method respectively. The contents of SOD CAT activity and MDA in left testicular tissues were detected by xanthine oxidase method and thiobarbituric acid method; Cell apoptosis index (AI) in cryptorchidism tissue was measured by TUNEL ; Morphology of testicular tissue was observed by HE staining. The expression of stem cell factor SCF was assessed by real-time quantitative PCR and western blotting.Results With the glial cell source sex nerve and fewer ten patients experienced septic complications, compared to (cryptorchidism + GDNF) have obvious change of each index: raw sperm cell apoptosis significantly reduced, superoxide dismutase (sod) rise, the content of mda drops, SCF gene and protein expression enhanced obviously, its significant difference , SOD CAT MDA were: 1.30±0.09; 288.78±10.02; 1.61±1.09 (P<0.01) the SCF gene and protein expression content respectively: 0.36±0.11, 0.48±0.23, 0.81±0.09, Group C compared with A, B, D between groups were significant statistical significance C vs A, B, D;A vs D; B vs D, (P<0.05). 0.61±0.33; 089±0.07, l.50±0.47, 2.19±0.12, 1.71±0.03 Group C compared with A, B, D between groups were significant statistical significance C vs A, B, D; A vs D; B vs D, (P<0.01).Conclusion Glial cell derived neurotrophic factor played an important role in the protection of rat unilateral cryptorchidism injury by antioxidant ability and upregulating of testicular stem cell factor, thus improve the testicular fertility.%目的：探讨小鼠隐睾模型睾丸损伤后，胶质细胞源性神经营养因子（glial cell derived neurotrophic factor，GDNF）对隐睾后睾丸组织中干细胞因子（stem cell factor SCF）及超氧化物歧化酶（SOD）、过氧化氢酶CAT、丙二醛（MDA）的影响。方法选取雄性鼠左侧隐睾成功模型48只，随机分为A、B、C、D4组（每组12只）：A组为隐睾组，B组为生理盐水（隐睾+生理盐水）组，C组为GDNF（隐睾+GDNF）组，D组为正常对照组（正常35d龄小鼠，体质量60g±10g）。每组根据设定处理方式的不同分别进行处理，采集左侧睾丸并行生化指标（SOD、CAT活性及MDA含量）检测；原位缺口末端标记（TUNEL）检测隐睾组织细胞凋亡指数（AI）；HE染色观察睾丸组织学形态；定时PCR法检测隐睾组织中SCF基因的表达情况，Western blot检测各组隐睾组织中SCF蛋白表达情况。结果 GDNF组中各项指标有明显变化：生精细胞凋亡明显减少，SOD活性上升，MAD含量下降，SCF基因及蛋白表达明显增强，其与各组比较，差异均有统计学意义，SOD、CAT、MDA分别为1.30±0.09，288.78±10.02和1.61±1.09（P＜0.01）。A、B、C、D组SCF基因表达含量分别为0.36±0.11，0.48±0.23，0.81±0.09和0.61±0.33；C组与A、B、D组；A组与D组；B组与D组比较均有统计学意义（P＜0.05）。A、B、C、D组SCF蛋白表达含量分别为0.89±0.07，l.50±0.47，2.19±0.12和1.71±0.03； C组与A、B、D组；A组与D组；B组与D组比较均有统计学意义（P＜0.01）。结论 GDNF对小鼠单侧隐睾睾丸损伤后组织生精功能具有保护和修复作用，同时提高抗氧化酶系统的抗氧化能力及睾丸SCF基因的表达含量，从而改善睾丸生育能力。
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