目的:探索神经性病理痛中星形胶质细胞被激活后的形态变化及相关蛋白的表达.方法:SD大鼠分为坐骨神经慢性结扎模型组(CCI组)和假手术组(Sham组),并于术前1d和术后1、3、5、7d取第4～5腰段脊髓做石蜡切片,免疫荧光组织化学标记胶质纤维酸性蛋白(GFAP)和波形蛋白的表达,后采用免疫荧光双标技术检测.结果:CCI组术后术侧脊髓背角GFAP免疫阳性细胞数量增多,体积变大,突起较长；波形蛋白免疫阳性细胞数量增多；GFAP和波形蛋白平均荧光强度明显增高.Sham组波形蛋白平均荧光强度术后较术前1d有所增强.波形蛋白和GFAP在CCI组脊髓背角术侧的分布有较好的一致性.结论:在神经病理性疼痛中,脊髓背角星形胶质细胞被激活,且呈现肥大和增殖两种病理学变化.%Objective: To investigate the morphological changes and expressions of associated proteins after astrocytes are activated in the neuropathic pain. Methods: SD rats were randomly divided into two groups: a chronic constriction injury of the sciatic nerve (CCI) model group and a sham operation group. No. 4-5 lumbar spinal cord was taken to make paraffin slices 1 day before operation and 1, 3, 5, 7 days after operation. The expression of glial fibrillary acidic protein (GFAP) and vimen-tin was labelled by immunofluorescence method, and then double immunofluorescent was stained with double immunolabel-ing techinque. Results: The GFAP positive cells in the (XI groups were increased in the number, larger in size, longer in process in comparing to the sham group. The vimentin positive cells were increased in the number. The mean fluorescence intensities of the GFAP and the vimentin were significantly different compared with others. The mean fluorescence intensity of the vimention began to increase in the sham operation groups after operation with statistical significance compared with the day before surgery. Vimentin and GFAP had a good consistence in operative side of the spinal dorsal horn in the CCI groups. Conclusion: In the neuropathic pain, astrocytes in spinal dorsal horn are activated and show pathological changes of proliferation and hypertrophy.
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