牛蒡子苷元对人膀胱癌T24细胞增殖、侵袭转移的影响

摘要

目的:研究牛蒡子苷元在体外对人膀胱癌细胞系T24细胞增殖、侵袭转移能力的影响.方法:培养T24细胞,采用不同浓度牛蒡子苷元处理,通过MTT、Transwell及划痕法观察牛蒡子苷元在不同时间点对T24细胞增殖、粘附、侵袭、迁移能力的影响；免疫印迹检测基质金属蛋白酶-9(MMP-9)蛋白的表达.结果:随着药物浓度升高(0～100μmol/L)和作用时间的延长(12～48 h),牛蒡子苷元抑制细胞增殖能力增强.牛蒡子苷元(40～100μmol/L)能明显抑制T24细胞的粘附能力.同时,牛蒡子苷元能够降低T24细胞的侵袭和迁移能力.随着作用浓度的增加,T24细胞的MMP-9蛋白表达明显降低.结论:牛蒡子苷元具有抑制人膀胱癌T24细胞增殖、粘附、侵袭和迁移的作用,且呈剂量依赖性,其作用可能与MMP-9的表达降低有关.%Objective:To investigate the effects of the arctigenin on proliferation, invasion and metastasis of human bladder cancer T24 cells in vitro. Methods: Human bladder cancer T24 cell lines were cultured in vitro; MTT assay was used to determine the inhibitive effect of arctigenin on the proliferation and adhesion of T24 cells. The invasion ability was assessed with Transwell chamber assay. Wound healing assay was used to investigate the migration ability. The role of matrix metal-loproteinase-9 (MMP-9) protein levels was analyzed by Western blotting. Results: MTT assay showed that the cell viability was obviously decreased with arctigenin treatment at different concentrations of 0-100 μmol/L in a dose-dependent and time-dependent manner. Arctigenin could decrease the adhesion activities of T24 cells in a dose-dependent manner. The cellular migration ability and invasion ability was also inhibited in a dose-dependent manner. Western blotting analysis indicated that the treatment of T24 cells with arctigenin showed in a dose-dependent (40-100 μmol/L) decrease in MMP-9 protein levels. Conclusion: arctigenin can significantly inhibit the proliferation, adhesion, invasion and migration ability of T24 cells in a dose-dependent manner. The inhibition of the invasion ability of T24 cells by arctigenin might be attributed to the decrease of MMP-9 expression. u0000e