溴代十六烷基三甲基铵敏化亮绿-脱氧核糖核酸作用的共振光散射增强研究

摘要

This is the first report of the sensitizing effect of cation surfactant on the enhanced resonance light scattering (RLS) resulted from the interaction of organic dyes with Deoxyribonucleic acid (DNA). On the conditions of pH 10.4 ～11.8 and ionic strength being less than 0.050, the interaction of brilliant green (BG) with DNA results in enhanced RLS signals at 398.0 and 467.2 nm overloaded some shoulder peaks in the enhanced region. It was found that the interaction was sensitized by the presence of cetyltrimethylammonium bromide (CTMAB). If 2.0～3.2×10-5 mol/L CMAB was used in the interacting medium, DNA in the range of 0～1.2 (mg/L could be detected with the limit of detection being less than 5 μg/L. Four synthetic samples were analyed with reproducibility%首次报道在表面活性剂存在下阳离子染料与脱氧核糖核酸(DNA)作用所导致的共振光散射增强(RLSE)。在pH 10.4 ～11.8 和离子强度低于0.050 mol/L的条件下，亮绿(BG)与DNA作用产生398.0 和 467.2 nm的特征RLSE信号，而溴代十六烷基三甲基铵(CTMAB)进一步敏化该RLSE信号。受CTMAB敏化的RLSE信号与0～1.2 mg/L的ctDNA和fsDNA呈正比，检测限低于5 mg/L。方法成功应用于合成样中DNA的测定。