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Cetyltrimethylammonium bromide gel electrophoresis
Cetyltrimethylammonium bromide gel electrophoresis
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机译:十六烷基三甲基溴化铵凝胶电泳
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摘要
A discontinuous polyacrylamide and agarose gel electrophoresis system is provided which allows the fine separation of proteins based on molecular weight with the concomitant retention of native enzymatic activity. This system, referred to as the CAT gel, uses the cationic detergent CTAB, and includes a stacking gel based on a zwitterion such as arginine and a buffer such as tricine. The CAT gel system allows specific enzyme histochemical detection and localization of proteins after gel electrophoresis. This system stacked and separated a broad range of proteins into discrete bands which migrate as a linear function of log M. sub.r. The effect of CTAB solubilization on the activity of several proteins is also shown. Proteins separated by CAT electrophoresis maintain high levels of native enzymatic activity, and may be detected histochemically in situ.
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机译:提供了一种不连续的聚丙烯酰胺和琼脂糖凝胶电泳系统,该系统可以根据分子量精细分离蛋白质,并同时保留天然酶活性。该系统称为CAT凝胶,使用阳离子清洁剂CTAB,并包括基于两性离子(如精氨酸)和缓冲液(如Tricine)的堆积凝胶。 CAT凝胶系统允许在凝胶电泳后进行特定的酶组织化学检测和蛋白质的定位。该系统将广泛的蛋白质堆叠并分离成离散的条带,这些条带作为log M. sub.r.的线性函数迁移。还显示了CTAB增溶对几种蛋白质活性的影响。通过CAT电泳分离的蛋白质可保持高水平的天然酶活性,并可通过组织化学原位检测。
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