ITO玻璃芯片上温度分布对成肌细胞生长增殖的影响

摘要

为了研究不同温度对小鼠成肌细胞生长增殖的影响,在ITO玻璃芯片上加载电场形成一定的温度分布.在不同温度(38、39、40和41℃)所对应的区域加工相同尺寸的PDMS微型培养腔室用于小鼠成肌细胞的培养.通过对芯片上培养的小鼠成肌细胞连续5d的热刺激(30 min/d),研究不同温度短期热刺激对成肌细胞增殖的影响.细胞形态显微观察和流式细胞仪检测结果表明,一定的温度刺激对小鼠成肌细胞的增殖有促进作用,其中40C刺激后的细胞数目增加最明显.在总刺激时间(30 min)相同情况下,短时多次热刺激的效果更理想,细胞增殖指数最高可达38.39.%In order to study the impact of different temperatures on the growth and proliferation of myoblasts,specific electric fields were loaded on indium-tin oxide (ITO) glass chips to form some temperature profiles.In several zones with different temperatures (38,39,40 and 41 ℃),microchambers with equal size were fabricated for the culture of mouse myoblasts.Impacts of temperatures on growth and proliferation of myoblasts were studied by stimulating cells for continuous 5 d (30 min/d).Microscopic observation of cell morphology and flow cytometric detection results showed that certain thermal stimulation could promote the proliferation of myoblasts.Number of cells was obviously increased after the stimulus of 40 ℃.For equal total stimulation time,short-term multiple thermal stimulation could achieve better effect and maximum proliferation index could reach 38.39.