超高压液相色谱-飞行时间质谱法对比分析沙丁胺醇在猪尿液和血浆中的代谢产物

摘要

采用高分辨质谱及代谢物鉴定软件，系统分析了猪尿液和血浆中沙丁胺醇代谢产物。样品用乙腈沉淀蛋白，经离心过滤后，采用Agilent Plus C18色谱柱，以乙腈和醋酸溶液(含0.2%醋酸)作为流动相进行梯度洗脱，采用高分辨质谱( UHPLC-QTOF MS)±离子模式进行检测，利用软件Agilent MassHunter MetaboliteID与人工相结合分析检测数据。结果表明，经灌胃给药后猪尿液中鉴定出沙丁胺醇原形药和葡糖醛酸化、苯环羟基化、N-氧化、苯环甲氧基化、羟基化脱水共5个代谢产物，其中2个代谢物未见文献报道；而血浆中仅检测原型药物和2个代谢物，包括沙丁胺醇的葡糖醛酸化和N-氧化产物。%Theexcretionofurinaryandplasmametabolitesofsalbutamolwasstudiedusingultrahigh performance liquid chromatography electrospray time-of-flight mass spectrometry, after a single intragastric gavaged dose administration with salbutamol. The software of Agilent MassHunter Metabolite ID was employed to find and identify the chemical structure of metabolites of salbutamol. Five metabolites from salbutamol were identified. Metabolites identified in swine urine included glucuronide conjugate salbutamol, N-oxide-salbutamol, hydroxyl-salbutamol, methoxyl-salbutamol and dehydrated hydroxyl-salbutamol. Whereas, only the parent drug, glucuronide conjugate salbutamol and dehydrated hydroxyl-salbutamol were observed in swine plasma.