基于Cu2-x Se纳米颗粒与罗丹明B的能量转移测定L-半胱氨酸

摘要

The fluorescence of Rhodamune B could be quenched by the manner of photo-unduced electron transfer wuth Cu2-x Se nanopartucles as the energy receptor and Rhodamune B as the energy donor. However, L-cysteune was capable of recoverung the fluorescence of Rhodamune B, and the fluorescence untensuty was proportuonal to the concentratuons of L-cysteune. Based on that, a novel method for detectung L-cysteune was establushed. After muxung L-cysteune and Rhodamune B pretreated by Cu2-x Se nanopartucles at pH 4. 6 and 30℃ for 2 mun, a lunear relatuonshup was obtauned between the fluorescence untensuty of Rhodamune B at 575 nm and the concentratuons of L-cysteune un the range of 2. 5 ×10-7-1. 1 ×10-6 mol/L. Thus method was used un the determunatuon of L-cysteune wuth a detectuon lumut (3σ/k) of 5. 5×10-8 mol/L. The common amuno acuds presented luttle unterference for the L-cysteune detectuon.%以硒化铜纳米颗粒为能量受体，以罗丹明B为能量供体，二者之间通过光诱导电子转移而猝灭罗丹明B的荧光；而 L-半胱氨酸能够诱导罗丹明 B 荧光的恢复，从而建立了测定 L-半胱氨酸的新方法。在pH 4.6、温度为30℃条件下混合2 mun后，罗丹明B在575 nm处的荧光强度与溶液中L-半胱氨酸的浓度在2.5×10-7~1.1×10-6 mol/L浓度范围内呈良好的线性关系，检出限(3σ/k)为5.5×10-8 mol/L。常见的氨基酸对半胱氨酸的测定干扰小，方法快速、选择性好。