间接顶空固相微萃取-气相色谱-质谱法对红花香雪兰天然花香成分的分析

摘要

A method was established for the analysis of red Freesia flower volatiles by indirect headspace solid phase microextraction ( HS-SPME) . The gas collecting tube was used to enrich flower volatiles, and adsorbing material was resolved in the extraction solution. Then, the adsorbing material was analyzed by HS-SPME. The experimental conditions for the quantification of major flower volatiles ( linalool, terpineol, ionone and dihydrogen-ionone) were optimized. Florisil was used as adsorbent, with water-methanol solution (9∶1, V/V) and 0. 6 mol/L hydrochloric acid as the extraction solution. The thermal desorption temperature was 70℃ and the extraction time was 25 min. The results showed that the concentration range of linear relationship for linalool and terpineol ( R2≥0. 981 ) was 1-100 μg/mL, with detection limits of 0. 05 and 0. 10 μg, respectively, and 0. 5-50 μg/mL for dihydro ionone and ionone ( R2≥0. 988 ) with a detection limit of 0. 02 μg.%建立了间接顶空固相微萃取-气相色谱-质谱对红花香雪兰天然花香成分的分析方法。首先利用气体采样装置对花香成分进行固相富集,富集后的花香成分在浸提液中解析,之后利用顶空固相微萃取方法对浸提液中的花香成分进行分析。红花香雪兰花香中主要成分(沉香醇、松油醇、紫罗兰酮和二氢紫罗兰酮)定量分析优化实验条件：Florisil(弗罗里硅土)作为吸附剂,水-甲醇混合液(9∶1, V/V)和0.6 mol/L HCl作为浸提液,萃取温度70℃,平衡时间为25 min。实验结果表明,沉香醇和松油醇在1~100μg/mL范围内有良好的线性关系(R2≥0.981),检出限分别为0.05和0.10μg；二氢紫罗兰酮和紫罗兰酮在0.5~50μg/mL范围内有良好的线性关系(R2≥0.988),检出限为0.02μg。