Fe3O4/MnO2掺杂石墨烯基分子印迹杂化材料用于水环境中17β-雌二醇的电化学传感检测

摘要

In this paper, Fe3O4/MnO2doped graphene molecularly imprinted hybrid material ( Fe3O4/MnO2-MIP@ RGO ) was successfully synthesized via reversible addition fragmentation chain transfer ( RAFT ) molecularly imprinting technique by using methacrylic acid as functional monomer, divinylbenzene as cross-linker, Fe3O4/MnO2@ RGO as carrier, and 17β-estradiol ( 17β-E2 ) as template molecule. A novel molecularly imprinting electrochemical sensor by using Fe3O4/MnO2-MIP@ RGO modified electrode was constructed to specifically detect trace 17β-E2 in water. The experimental results showed that the Fe3O4/MnO2-MIP@ RGO electrochemical sensor exhibited rapid and linear current response to 17β-E2 in water samples with a linear range of 4 nmol/L to 0. 8 μmol/L ( R=0. 9852) , the detection limit was 47. 2 pmol/L (3σ) and the relative standard deviation (RSD) was from 2. 1% to 2. 5% . This study provides a simple and efficient, economical and reliable method for the monitoring of 17β-estradiol in the complex water environment.%采用可逆加成-断裂链转移( Reversible addition fragmentation chain transfer, RAFT)分子印迹技术,以内分泌干扰物17β-雌二醇(17β-estradiol, 17β-E2)作为模板分子,以甲基丙烯酸( Methacrylic acid, MAA)为功能单体,二乙烯基苯(Divinyl benzene, DVB)为交联剂,Fe3O4/MnO2掺杂的石墨烯(RGO)为载体,成功制备了Fe3O4/MnO2掺杂石墨烯基分子印迹杂化材料( Fe3O4/MnO2-MIP@RGO),构建了基于 Fe3O4/MnO2-MIP@RGO修饰电极的分子印迹电化学传感器.研究结果表明,Fe3O4/MnO2-MIP@RGO修饰电极对17β-E2的线性响应范围为4.0 nmol/L~0. 8 μmol/L(R=0. 9852),检出限为47. 2 pmol/L(3σ),相对标准偏差(RSD)为2. 1% ~2. 5% .本研究为水环境中痕量17β-E2的特异性识别检测提供了一种简单、高效、经济的分析方法.