聚羟基丁酸酯/聚乙二醇纳米复合支架的生物相容性研究

摘要

ObjectiveThree-dimensional nano composite biological scaffolds were prepared using polyhydroxybutyrate (PHB)/polyethylene glycol(PEG) and compound culture with rat condylar cartilages, to evaluate its biocompatibility.Methods The PHB/PEG nano composite biological scaffolds were prepared by electrospinning and the rat condylar cartilages were seeded in it. The adhesion, growth and spreading of cells were observed by lfuorescence microscope and scanning electron microscope after seeding 4h and 72h, and evaluated cells' proliferation and scaffold biocompatibility using MTT.Results From the results of lfuorescence microscope and scanning electron microscope, cells adhere on the scaffolds completely and some cells began spreading after 4h, the proliferation and growth were better after 72h. The results of MTT showed that PHB/PEG nanocomposite scaffolds can promote proliferation of rat condylar cartilages.ConclusionThe PHB/PEG nanocomposite biological scaffolds have good biocompatibility and can promote adhesion, growth and proliferation of rat condylar cartilages, which will establish on experimental basis for tissue engineering cartilage regeneration.%目的:采用聚羟基丁酸酯(polyhydroxybutyrate,PHB)/聚乙二醇(polyethylene glycol,PEG)纳米复合生物材料构建三维培养支架并与大鼠髁突软骨细胞(condylar chondrocytes)复合培养,评价其生物相容性.方法:应用静电纺丝法制备PHB/PEG纳米复合生物支架,将大鼠髁突软骨细胞接种于PHB/PEG支架上,分别于复合培养4h,72h时利用荧光显微镜及扫描电子显微镜观察细胞在三维支架上的粘附、铺展及生长情况,并应用MTT四甲基偶氮唑盐微量酶反应比色法检测细胞的增殖活性,评价其生物相容性.结果:荧光显微镜及扫描电子显微镜观察显示复合培养4h时细胞已粘附于支架表面,部分细胞开始铺展,培养72h细胞在支架表面增殖及生长良好,MTT结果显示PHB/PEG纳米三维支架复合培养大鼠髁突软骨细胞较对照组具有更高的增殖活性.结论:静电纺丝法制备的PHB/PEG纳米复合生物支架可以促进大鼠髁突软骨细胞的粘附、生长和增殖,具有良好生物相容性,为组织工程软骨再生奠定一定的实验基础.