以8个核桃品种叶片为材料，利用ISSR技术，从100条IssR引物中筛选出2条对8份DNA进行PCR扩增，共扩增位点54个，其中多态位点24个（比例达70．58％），构建出8个核桃品种的数字化指纹图谱。以NTSYSpc2．1软件计算品种指纹图谱的相似系数（Gs）并进行（UPGMA）聚类分析，样品间的Gs在0．54～0．94之间，在GS=0．64处将8个核桃品种分成2组：第一组母本为‘扎345’（1号），矮化品种有‘新温72．4’（4号）和‘新温908’（5号）；第二组母本为‘新早丰’（2号），矮化品种有‘新温609’（5号）、‘新温915’（6号）、‘新温916’（7号）和‘新温917’（8号），与田间表现基未一致．%PCR amplification was conducted on the 8 DNA derived from fresh leaves of eight walnut cultivars with two primers which were selected from 100 ISSR primers. The results showed that 34 loci were detected, among which there were 24 polymorphic loci, and the sample have polymorphic ratio of 70.58%. Based on the statistical data of the electrophoresis patterns, digital fingerprint of eight walnuts cultivars was established. The genetic similarities （GS） of walnut cultivars were calculated by NTSYSpc 2.1 software and then UPGMA cluster analysis was conducted. The genetic similarities （GS） among cultivars ranged from 0.54 to 0.94. When GS was 0.64, the eight walnut cultivars could be clustered in to two groups. The female parent of 343 （1） , dwarf cultivar of Xinwen724 （4） and Xinweng08 （5） were in the first group; the second group included female parent of Xinzaofeng （2） , dwarf cultivar of Xin- wen609 （3） , Xinwen915 （6） , Xinwen916 （7） and Xinwen917 （8） . the field walnut results basically were consistent with result of UPGMA cluster.
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