6种杀菌剂对枇杷灰斑病的室内毒力测定

摘要

The pathogenic bacteria of loquat gray spot was separated, purified and identified. Then the toxicity and field control effects of 6 bactericides against loquat gray spot were tested by the indoor fungicide test to provide a reference for prevention and cure of loquat gray spot. The results showed that pathogenic bacterium was Pestalotiopsis eriobotryfolia (Guba) in Guizhou. All these fungicides could inhibit the mycelial growth to a varying extent in a dosage dependent manner. Among these 6 fungicides, 80%Tebuconazole WDC had the best effect [EC50 value:5.69μg/ml (r=0.9937)], and then followed by 80%Propineb WP, 40%Myclobutanil SC, 325 g/L Difenoconazole·Azoxystrobin SC, 400 g/L Flusilazole EC and 500 g/L Benzoyl Propiconazole EC.%为了给枇杷灰斑病田间防治提供理论依据，试验通过对来源于贵州省部分地区枇杷种植园的灰斑病病原菌分离物进行分离、纯化和鉴定，并选用6种杀菌剂进行室内毒力测定。结果表明，贵州地区枇把灰斑病致病菌为拟盘多毛孢[Pestalotiopsis eriobotryfolia (Guba)]；6种杀菌剂对批把灰斑病菌的菌丝生长的抑制率与浓度呈正相关，其中80%戊唑醇WDG的抑菌效果最好，EC50为5.69μg/ml (r=0.9937)，其次分别为80%丙森锌WP、40%腈菌唑SC、325 g/L苯甲嘧菌酯SC、400 g/L氟硅唑EC和500 g/L苯甲丙环唑EC。