对虾白斑杆状病毒（WSBV）定量PCR检测技术研究

摘要

A DNA fragment of White Spot Bac illiform Virus which is lack of 74 bp was preperred and used as a competi tive internal sta ndard DNA which is amplified with the positive template or infected shrimp DNA tem plate to study its recovery and determine the content of WSBV in the infected sh rimp. The results showed that the sensitivity of the quantitave PCR reach to the Virus DNA of 10 molecular/μl. The recovery was estimated at about 50%.%报道了利用DNA重组技术在一段对虾白斑杆状病毒（White Spot Bacilliform Viru s,, WSBV）的基因组DNA片段中缺失74bp，重组得到的内标质粒作为竞争性内标模板，并与 阳标模板或病虾DNA模板共同扩增，建立了定量PCR检测方法。研究表明，定量PCR方法检测 灵敏度达10病毒分子DNA/μl，样品DNA模板制备回收率约50％，并能对病虾样品中的WSBV含 量进行定量分析。