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Identification, purification and detection of WSBV (baculovirus associated with white spot syndrome)

机译:鉴定,纯化和检测WSBV(与白斑综合症有关的杆状病毒)

摘要

This invention relates to the identification, purification and detection of a new infectious viral agent in arthropods, especially shrimps. The virus is named as WSBV (Baculovirus associated with white spot syndrome). Two WSBV genomic DNA libraries were constructed and based upon the sequence of one of the cloned WSBV DNA fragments, a WSBV specific primer set for PCR to detect the WSBV infection in penaeid shrimps has been developed. The results of the present invention provide an effective diagnostic tool for screening WSBV infection in animal host organisms, in particular shrimps, to prevent the further spread of this viral disease.
机译:本发明涉及节肢动物尤其是虾中新型传染性病毒剂的鉴定,纯化和检测。该病毒被称为WSBV(与白斑综合症有关的杆状病毒)。构建了两个WSBV基因组DNA文库,并基于克隆的WSBV DNA片段之一的序列,开发了用于PCR的WSBV特异性引物组,用于检测对虾中WSBV的感染。本发明的结果提供了用于筛选动物宿主生物特别是虾中的WSBV感染的有效诊断工具,以防止该病毒疾病的进一步传播。

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