Runx3过表达对慢性乙型肝炎患者Th1、Th2型细胞因子表达的影响

摘要

Objective To investigate the effect of Runx3 overexpression on the expression of Th1 ,Th2-type cytokines in patients with chronic hepatitis B (CHB).Methods Peripheral CD4 +T cells derived from 29 CHB patients were transfected with the recombinant lentiviral vector (pGC-FU-Runx3)and the negative control lentiviral vector (pGC-FU),respectively.Cell culture supernatants were collected on day 3,5 and 7.The expression of Th1-type cytokines (IFN-γ,IL-2 )and Th2-type cytokines (IL-4,IL-10 )was measured by enzyme-linked immune sorbent assay (ELISA ), respectively.Results (1 )Compared with that in pGC-FU transfected group,the expression of IFN-γ in pGC-FU-Runx3 transfected group significantly increased on day 3 (P <0.05),5 (P <0.01)and 7 (P <0.01),respectively.Furthermore, the expression of IL-2 in pGC-FU-Runx3 transfected group significantly increased on day 5 (P <0.05)and 7 (P <0.01 ). However,there was no difference in IL-2 expression between pGC-FU transfected group and pGC-FU-Runx3 transfected group on day 3 (P >0.05).(2)Compared to that in pGC-FU transfected group,the expression of IL-4 in pGC-FU-Runx3 transfected group significantly decreased on day 5 (P <0.01 )and 7 (P <0.05 ).Nevertheless,there was no difference in IL-4 expression between pGC-FU transfected group and pGC-FU-Runx3 transfected group on day 3 (P >0.05).Moreover, the expression of IL-10 in pGC-FU-Runx3 transfected group significantly decreased on day 5 (P <0.05)and 7 (P <0.05). Nonetheless,there was no difference in IL-10 expression between pGC-FU transfected group and pGC-FU-Runx3 transfected group on day 3 (P >0.05).(3)Compared with that in pGC-FU transfected group,the ratio of IFN-γ/IL-4 in pGC-FU-Runx3 transfected group significantly increased on day 3 (P < 0.01 ),5 (P < 0.01 )and 7 (P < 0.01 ), respectively.Conclusion Runx3 overexpression could promote the secretion of Th1-type cytokines while inhibiting the secretion of Th2-type cytokines in CHB patients,which could improve the Th1/Th2 imbalance in CHB patients.%目的：探讨 Runx3过表达对 CHB 患者 Th1、Th2型细胞因子表达水平的影响。方法重组慢病毒载体pGC-FU-Runx3与阴性对照慢病毒载体 pGC-FU 分别转染29例 CHB 患者外周血 CD4＋ T 细胞，收集培养3 d、5 d 和7 d的细胞培养上清液，应用 ELISA 检测 Th1型细胞因子 IFN-γ、IL-2和 Th2型细胞因子 IL-4、IL-10的表达水平。结果与pGC-FU 转染组比较，pGC-FU-Runx3转染组 Th1型细胞因子 IFN-γ的表达水平在3 d（P ＜0．05）、5 d（P ＜0．01）和7 d （P ＜0．01）时均明显升高；IL-2的表达水平在3 d 时差异无统计学意义（P ＞0．05），但在5 d（P ＜0．05）和7 d（P ＜0．01）时均明显升高。与 pGC-FU 转染组比较，pGC-FU-Runx3转染组 Th2型细胞因子 IL-4的表达水平在3 d 时差异无统计学意义（P ＞0．05），但在5 d（P ＜0．01）和7 d（P ＜0．05）时均明显降低；IL-10的表达水平在3 d 时差异无统计学意义（P ＞0．05），但在5 d（P ＜0．05）和7 d（P ＜0．05）时均明显降低。与 pGC-FU 转染组比较，pGC-FU-Runx3转染组 IFN-γ／IL-4比值在3 d（P ＜0．01）、5 d（P ＜0．01）和7 d（P ＜0．01）时均明显增大。结论 Runx3过表达可以促进 CHB 患者 Th1型细胞因子的分泌，抑制 Th2型细胞因子的分泌，使其 Th1／Th2失平衡得到改善。