Objective To observe the dynamic changes of serum procalcitonin (PCT), tumor necrosis factor-α (TNF-α) and interleukin-8 (IL-8) in patients with acute pancreatitis (AP) and to investigate their significance in judging AP severity and the occurrence of concurrent infection. Methods 56 AP patients admitted to the First Affiliated Hospital of Suzhou University and Kunshan Hospital of TCM from October 2009 to October 2011 were selected. Among the patients, 21 cases were mild acute pancreatitis (MAP, MAP group) and 35 cases were severe acute pancreatitis (SAP, SAP group) . 5 patients in SAP group combined with pancreatic infection were involved into infection group and other 30 patients were involved into non-infection group. Serum PCTs on admission, the third day after admission, he seventh day after admission were determined by EL-MA and TNF-α and IL-8 were determined by ELISA. Results The levels of serum PCT, TNF-α and IL-8 in the SAP group were all significantly higher than those in the MAP group (P<0. 01) . The levels of serum PCT, TNF-α and IL-8 reached the peak level on the third day after admission in the two groups, but their levels in the SAP group were still significantly higher than those in the MAP group (P<0. 05) . The levels of serum PCT, TNF-α and IL-8 both reduced on the seventh day after admission, but their levels in the SAP group were still significantly higher than those in the MAP group (P<0. 05) . The levels of serum PCT on admission, the third day after admission and the seventh day after admission in the infection group were all significantly higher than those of the non-infection group (P<0. 01) . The levels of TNF-α and IL-8 on admission, the rnthird day after admission and the seventh day after admission in the infection group were all higher than those of the non-infection group but without statistical significance (P > 0. 05) . Conclusion TNF-α and IL-8 can be used for the diagnosis of SAP and MAP, but can not be used to judge whether there is infection. PCT can not only reflect the severity of AP, but can also judge whether there is infection at an early stage, helping choose antibody reasonably at an early stage and prevent infection. Serum PCT, TNF-α and IL-8 can be easily determined with good accuracy and low price. They can also be dynamically monitored, which is conducive to choosing more reasonable treatment by the doctor.%目的 观察急性胰腺炎(AP)患者降钙素原(PCT)、肿瘤坏死因子α(TNF-α)及白介素8(IL-8)的动态变化,探讨其判断AP病情程度及有无合并感染的意义.方法 选择苏州大学附属第一医院和昆山市中医医院2009年10月-2011年10月AP患者56例,其中轻症急性胰腺炎(MAP)患者21例(MAP组),重症急性胰腺炎(SAP)患者35例(SAP组);以SAP组合并胰腺感染患者5例为感染组,非感染者30例为非感染组.采用双抗体夹心免疫发光法检测患者入院时、第3天、第7天血清PCT水平,酶联免疫吸附法检测血清TNF-α和IL-8水平.结果 入院时SAP组血清PCT、TNF-α及IL-8水平均高于MAP组,差异均有统计学意义(P＜0.01);入院第3天两组血清PCT、TNF-α及IL-8水平达到高峰,SAP组血清PCT、TNF-α及IL-8水平仍高于MAP组,差异均有统计学意义(P＜0.05);入院第7天两组血清PCT、TNF-α及IL-8水平有所下降,但SAP组血清PCT、TNF-α及IL-8水平仍高于MAP组,差异均有统计学意义(P＜0.05).感染组血清PCT水平在入院时、第3天、第7天均高于非感染组,差异有统计学意义(P＜0.01);感染组血清TNF-α和IL-8水平在入院时、第3天、第7天也高于非感染组,但差异均无统计学意义(P＞0.05).结论 TNF-α和IL-8可用以诊断SAP和MAP,但均不能用以判断是否合并感染.PCT不仅能在早期反映AP病情程度,还可以早期判断是否合并感染,有助于早期合理选择抗生素与预防感染.血清PCT、TNF-α及IL-8检测方法简单易行,准确度高,价格低廉,还可进行动态监测,有助于指导临床医生做出更合理的治疗.
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