OX40配体高表达在慢性心力衰竭患者中的临床意义

摘要

目的：探讨慢性心力衰竭患者血清可溶性OX40配体(sOX40L)高表达的临床意义。方法：应用酶联免疫吸附法(ELISA)分别对正常对照组30例、慢性心力衰竭组50例的血清sOX40L水平进行检测；应用脑钠肽(BNP)试剂盒放射免疫法检测两组血清BNP；采用多普勒心脏超声检测两组左室射血分数(LVEF)。结果：(1)慢性心力衰竭组血清sOX40L水平明显较对照组高，差异有统计学意义(P<0.01)；(2)慢性心力衰竭组BNP水平明显较对照组高，差异有统计学意义(P<0.01)；(3)慢性心力衰竭组LVEF水平明显较对照组低，差异有统计学意义(P<0.01)；而且血清sOX40L 水平与BNP呈显著正相关(r=0.69，P<0.001)，与LVEF呈显著负相关(r=0.53，P<0.001)。结论：慢性心力衰竭患者血清sOX40L和BNP水平均明显升高；血清sOX40L水平与BNP呈正相关，提示sOX40L高表达在慢性心力衰竭患者早期诊断中有良好的预警作用。%Objective:To investigate the clinical implications of increased expression of soluble OX40 ligand (sOX40L) in serum in patients with chronic heart failure(CHF).Method:30 control and 50 patients with chronic heart failure were enrolled in this study.Expression of sOX40L levels were measured by enzyme linked immunosorbent assay (ELISA).Brain natriuretic peptide(BNP)in serum was determined by radioimmunoassay.Left ventricular ejection fraction(LVEF) was measured with ultrasound cardiography.Result:(1)The serum levels of sOX40L in patients with chronic heart failure were significantly higher than control,the difference was statistically significant(P<0.01).(2)BNP in serum in patients with chronic heart failure were significantly higher than control group,the difference was statistically significant(P<0.01).(3)LVEF in patients with chronic heart failure were significantly lower than control group,the difference was statistically significant(P<0.01).The level of sOX40L had a positive correlation with BNP level(r=0.69,P<0.01),it negatively correlated with LVEF(r=0.53,P<0.001). Conclusion:Expression of sOX40L serum concentration and BNP level are both elevated in patients with chronic heart failure.BNP level is related to sOX40L in se rum.It suggestes that enhances level of serum sOX40L can make a good forewarning role in the early diagnosis of CHF.