目的：对生物芯片化学发光免疫法在检测人TSH和T4中的价值进行评价分析，为今后的临床研究工作提供可靠的参考依据。方法建立生物芯片化学发光免疫法，采集15例患者血清展开TSH和T4水平检测，并对测定结果进行统计分析。结果所建立的生物芯片化学发光免疫法检测TSH的灵敏度为0.096mIU/L，T4的灵敏度为4.5ng/ml，线性关系良好，TSH的回收率在91%-101%之间，T4的回收率在92%-103%之间，检测结果准确。结论采取生物芯片化学发光免疫法对人体血清中TSH和T4水平进行检测时具有较高的灵敏度、线性关系良好，回收率与准确性高，值得关注并推广。%objective to biochip, chemiluminescence immunoassay in detecting TSH and makes an analysis of the value of T4, provide reliable reference for future clinical research work. Method to establish the biological chip, chemiluminescence immunoassay, col ection of 15 patients with serum TSH and T4 level detection, and the measurement results were analyzed. Results of biochip, chemiluminescence immunoassay detection is 0.096 mIU/L of the sensitivity of TSH T4 sensitivity of 4.5 ng/ml, good linear relationship, the recovery rate of TSH between 91% and 101%, the recovery rate of T4 between 92% and 103%, test results are accurate. Conclusion the method of biochip chemiluminescence immune detecting a human serum TSH and T4 levels with high sensitivity, good linearity, high recovery rate and accuracy, worthy of attention and promotion.
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