宫颈活检标本病理诊断中P16和ki-67免疫组化染色的联合应用

摘要

Objective:To investigate the effect of combined application of P16 and ki-67 immunohistochemical staining in the diagnosis of cervical biopsy specimens.Methods:90 patients with cervical lesion who had cervical biopsy were selected.We analyzed the data of them retrospectively.25 patients with normal or had inflammation were set as the control A group;15 cases of patients with squamous cell carcinoma of the uterine cervix design for the control B group;50 patients with squamous intraepithelial lesions as the study group.The three groups were detected the expression of p16 and ki-67 by immuno histochemistry.Results:The total positive rate of the control A group was lower than that of the control B group and the study group, and the total positive rate of the study Ⅰ group was lower than that of the control B group(P<0.05);The total positive rate of the control A group was lower than that of the control group B and the study group(P<0.05).Conclusion:The combined application of P16 and ki-67 can effectively improve the accuracy of cervical lesion classification,which is helpful for clinical differentiation of cervical cancer and non neoplastic hyperplasia.%目的：探讨宫颈活检标本病理诊断中P16和ki-67免疫组化染色的联合应用效果。方法：收治因宫颈病变中予以宫颈活检的患者90例，对其资料进行回顾性分析，将其中25例正常或炎症患者设为对照A组，15例宫颈鳞癌患者设为对照B组，50例鳞状上皮内病变患者设为研究组，3组均予以免疫组化进行检测P16和ki-67表达。结果：对照A 组总阳性率比对照 B 组、研究组低，且研究Ⅰ组总阳性率比对照 B 组低(P＜0.05)；对照 A 组总阳性率比对照 B 组、研究组低(P＜0.05)。结论：P16与ki-67联合应用可有效提高宫颈病变分级准确性，有利于临床鉴别宫颈肿瘤性增生与非肿瘤性增生。